Abstract: There are provided an evaluation method for selecting a bisulfite reagent according to a target of a genetic test and a genetic test method including selecting a bisulfite reagent according to a target of a genetic test. In the evaluation method for a bisulfite reagent, a rate 1 of an error in which an unmethylated CpG site is determined to be in a methylated state is compared with a rate 2 of an error in which a methylated CpG site is determined to be in an unmethylated state. In the genetic test method, the bisulfite reagent is selected according to a history of illness of a subject and a correlation between a disease and methylation or unmethylation of a gene.

Abstract: There are provided a cancer examination reagent set which increases a reliability degree of a cancer examination and a production method for a cancer examination reagent set, as well as a cancer examination method having a high reliability degree. The cancer examination reagent set includes a primary examination reagent for examining n kinds of cancers and a secondary examination reagent for discriminating two kinds of the n kinds of cancers. The production method for cancer examination reagent set includes selecting a CpG site set 1 for examining n kinds of cancers and selecting a CpG site set 2 for discriminating two kinds among the n kinds of cancers. In the cancer examination method, two kinds of cancers having a high degree of certainty are selected from cancers detected by a primary examination, and the kinds of cancer are discriminated by a secondary examination.

Abstract: There is provided a method of calculating a base methylation degree from DNA sequence analysis data, which is a calculation method for a base methylation degree, including correcting a read based on quality information of sequence analysis data in a case of using a co-methylation site, using a paired-end read, using a molecular barcode, or using a plurality of sequence analysis data. There is also provided a program for causing a computer to execute the calculation method for a base methylation degree.