Abstract: A specimen image registration according to the invention includes selecting at least one third image different from a first image and a second image which are target images from the plurality of specimen images, obtaining a registration amount between the first image and the third image and a registration amount between the second image and the third image, and obtaining a registration amount between the first image and the second image based on the registration amount between the first image and the third image and the registration amount between the second image and the third image. It is possible to improve a success rate of registration even if the specimen images obtained by a plurality of types of staining are largely different in stained state.

Abstract: An image processing using a plurality of specimen images obtained by successively applying a plurality of types of staining to a specimen to be evaluated and imaging the specimen after staining for at least two types of staining is performed. The image processing comprises: extracting an inner region corresponding to inward of a cell membrane of a single cell in the specimen based on at least one of the specimen images, specifying a cell region corresponding to an individual cell included in the specimen by expanding the inner region outwardly, and performing image cytometry for the cell region based on the specimen images. In analyzing a pathological specimen on a cell-by-cell basis, it is possible to deal with multiple immunostaining and specify the positions of individual cells and evaluate each cell separately even when a cell density in the specimen is high.