Abstract: A cell culturing method and device that uses a cell culture bag (1) that includes a bag body (2) configured from an upper surface film (21) and a lower surface film (22) having a sealed periphery, and a port (3) attached to the bag body (2), and a plurality of recesses (4) being formed in the lower surface film (22). The method including: a closing-off step for closing off the plurality of recesses (4) using the upper surface film (21) by discharging a culture medium (S) contained in the bag body (2) through the port (3); and a releasing step for releasing, in some or all of the plurality of closed-off recesses (4), cells or cell aggregates adhering to the inner surfaces of the recesses (4).

Abstract: A cell culturing method using a cell culturing bag (1) in which a plurality of recess portions are formed, including: a bag body (2) configured from an upper film (21) and a lower film (22) which seal a peripheral portion; and a port (3) mounted on the bag body (2), wherein the cell culturing bag (1) is placed on a placement surface (5a) of a stand (5), and while a culture medium (S) is being injected or discharged from the port (3), a pressing member (6) having a bottom surface (6a) substantially parallel to the placement surface (5a) is used to press the upper film (21) from above.