Patents by Inventor Makoto Asashima
Makoto Asashima has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210322513Abstract: As a technique for specifically detecting cancer cells, provided is a method for detecting cancer cells, including the steps of: bringing BC2LCN lectin into contact with a test sample; and determining the presence or absence or the amount of a sugar chain having a BC2LCN lectin binding activity in the test sample, in which the test sample is a body fluid sample of a test individual.Type: ApplicationFiled: June 30, 2021Publication date: October 21, 2021Inventors: Hiroaki TATENO, Jun HIRABAYASHI, Makoto ASASHIMA, Yuzuru ITOU, Yasuko ONUMA, Tatsuya ODA, Nobuhiro OHKOHCHI, Osamu SHIMOMURA
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Publication number: 20180250360Abstract: As a technique for specifically detecting cancer cells, provided is a method for detecting cancer cells, including the steps of: bringing BC2LCN lectin into contact with a test sample; and determining the presence or absence or the amount of a sugar chain having a BC2LCN lectin binding activity in the test sample, in which the test sample is a body fluid sample of a test individual.Type: ApplicationFiled: October 5, 2016Publication date: September 6, 2018Inventors: Hiroaki TATENO, Jun HIRABAYASHI, Makoto ASASHIMA, Yuzuru ITOU, Yasuko ONUMA, Tatsuya ODA, Nobuhiro OHKOHCHI, Osamu SHIMOMURA
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Patent number: 9914908Abstract: An object of the present invention is to provide a method for introducing a target substance into undifferentiated cells, and a carrier therefor, whereby the target substance can be specifically introduced into the undifferentiated cells by contacting the undifferentiated cells with an rBC2LCN-target substance fusion product in which rBC2LCN lectin is fused to the target substance. Particularly, an rBC2LCN-toxin fusion product in which rBC2LCN lectin is fused to a toxin functioning in cells or its domain having the ability to kill cells functions as an agent for eliminating undifferentiated stem cells and can be administered into a medium after inducing the differentiation of the stem cells to reliably kill only the stem cells in an undifferentiated state.Type: GrantFiled: February 13, 2014Date of Patent: March 13, 2018Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Hiroaki Tateno, Yuzuru Ito, Yasuko Onuma, Jun Hirabayashi, Makoto Asashima
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Publication number: 20160369349Abstract: [Problem to be Solved] To provide a new lung-cancer biomarker, which has a high detection efficiency and determines the type (histological type) of lung cancer. [Solution] LIPH (lipase, member H), which is enhanced in a plurality of lung cancer-derived cell lines compared to that in normal lung-derived epithelial cells and is almost surely expressed highly in lung cancer.Type: ApplicationFiled: March 18, 2014Publication date: December 22, 2016Inventors: Yasuhiro SEKI, Norio SHIBATA, Makoto ASASHIMA, Akira KURISAKI
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Patent number: 9500650Abstract: An object of the present invention is to provide a method for evaluating a differentiation status of cells using a culture supernatant of stem cells. Provided is “undifferentiation sugar chain marker” composed of the sugar chain structure “Fuc?1-2Gal?1-3GlcNAc” or “Fuc?1-2Gal?1-3GalNAc” and capable of sensitively determining the undifferentiated state of stem cells using a culture supernatant of stem cells. Also found is BC2LCN lectin or a modified product thereof capable of sensitively recognizing the “undifferentiation sugar chain marker” as excellent “probe for detecting the undifferentiation sugar chain marker” capable of determining the undifferentiation status of cells using a culture supernatant.Type: GrantFiled: October 31, 2012Date of Patent: November 22, 2016Assignees: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventors: Hiroaki Tateno, Jun Hirabayashi, Yuzuru Ito, Yasuko Onuma, Makoto Asashima, Atsushi Kuno, Masaki Warashina, Masakazu Fukuda
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Patent number: 9279809Abstract: Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell.Type: GrantFiled: February 27, 2013Date of Patent: March 8, 2016Assignees: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventors: Hiroaki Tateno, Jun Hirabayashi, Makoto Asashima, Yuzuru Ito, Yasuko Onuma, Masaki Warashina, Masakazu Fukuda
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Publication number: 20150376568Abstract: An object of the present invention is to provide a method for introducing a target substance into undifferentiated cells, and a carrier therefor, whereby the target substance can be specifically introduced into the undifferentiated cells by contacting the undifferentiated cells with an rBC2LCN-target substance fusion product in which rBC2LCN lectin is fused to the target substance. Particularly, an rBC2LCN-toxin fusion product in which rBC2LCN lectin is fused to a toxin functioning in cells or its domain having the ability to kill cells functions as an agent for eliminating undifferentiated stem cells and can be administered into a medium after inducing the differentiation of the stem cells to reliably kill only the stem cells in an undifferentiated state.Type: ApplicationFiled: February 13, 2014Publication date: December 31, 2015Inventors: Hiroaki Tateno, Yuzuru Ito, Yasuko Onuma, Jun Hirabayashi, Makoto Asashima
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Publication number: 20150204870Abstract: An object of the present invention is to provide a method for evaluating a differentiation status of cells using a culture supernatant of stem cells. Provided is “undifferentiation sugar chain marker” composed of the sugar chain structure “Fuc?1-2Gal?1-3GlcNAc” or “Fuc?1-2Gal?1-3GalNAc” and capable of sensitively determining the undifferentiated state of stem cells using a culture supernatant of stem cells. Also found is BC2LCN lectin or a modified product thereof capable of sensitively recognizing the “undifferentiation sugar chain marker” as excellent “probe for detecting the undifferentiation sugar chain marker” capable of determining the undifferentiation status of cells using a culture supernatant.Type: ApplicationFiled: October 31, 2012Publication date: July 23, 2015Inventors: Hiroaki Tateno, Jun Hirabayashi, Yuzuru Ito, Yasuko Onuma, Makoto Asashima, Atsushi Kuno, Masaki Warashina, Masakazu Fukuda
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Publication number: 20150111218Abstract: Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell.Type: ApplicationFiled: February 27, 2013Publication date: April 23, 2015Applicants: National Institute of Advanced Industrial Science and Technology, Wako Pure Chemical Industries, Ltd.Inventors: Hiroaki Tateno, Jun Hirabayashi, Makoto Asashima, Yuzuru Ito, Yasuko Onuma, Masaki Warashina, Masakazu Fukuda
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Patent number: 8158423Abstract: A method of making a hair, including a step of culturing an undifferentiated cell of a mammal to produce an embryoid body and a step of further culturing the embryoid body is provided, wherein the culturing step is to culture the embryoid body on a three-dimensional matrix for 5 to 12 days. Furthermore, a biological material obtainable by the method of making a hair as described above is provided. Moreover, a biological material for a screening system of evaluating a medical product or the like, obtainable by utilizing the method of making a hair as described above is provided.Type: GrantFiled: February 5, 2008Date of Patent: April 17, 2012Assignee: Matsumoto Dental UniversityInventors: Hidehiro Ozawa, Mariko Yamaki, Makoto Asashima, Satoshi Ebina
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Patent number: 8105833Abstract: A method for forming an organ and/or tissue from undifferentiated cells derived from a vertebrate animal in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid X receptor ligand (e.g., a retinoic acid X receptor agonist or antagonist), and a method for forming a pancreas from undifferentiated cells derived from a vertebrate animal in vitro or a method for forming a tissue having morphology and function of a pancreas from undifferentiated cells derived from a vertebrate in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid receptor ligand, together with activin, that does not substantially bind to the retinoic acid receptor subtype ?.Type: GrantFiled: August 4, 2009Date of Patent: January 31, 2012Assignee: Research Foundation Itsuu LaboratoryInventors: Makoto Asashima, Tatsuo Hamazaki, Hiroyuki Kagechika, Koichi Shudo
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Patent number: 7923245Abstract: The present invention discloses a medium for a serum-free medium capable of culturing ES cells for a long period while maintaining their undifferentiated state without using feeder cells, and a basal medium for producing the medium thus described. The basal medium of the present invention is characterized by that it has composition shown by Table I. Further, the present invention discloses a medium for ES cells produced with the basal medium.Type: GrantFiled: December 27, 2004Date of Patent: April 12, 2011Inventors: Miho Furue, Tetsuji Okamoto, Makoto Asashima
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Publication number: 20100304479Abstract: A method of making a hair, including a step of culturing an undifferentiated cell of a mammal to produce an embryoid body and a step of further culturing the embryoid body is provided, wherein the culturing step is to culture the embryoid body on a three-dimensional matrix for 5 to 12 days. Furthermore, a biological material obtainable by the method of making a hair as described above is provided. Moreover, a biological material for a screening system of evaluating a medical product or the like, obtainable by utilizing the method of making a hair as described above is provided.Type: ApplicationFiled: February 5, 2008Publication date: December 2, 2010Inventors: Hidehiro Ozawa, Mariko Yamaki, Makoto Asashima, Satoshi Ebina
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Publication number: 20100015199Abstract: The present invention provides a method for suppressing generation of a teratoma of an undifferentiated cell, preferably an ES cell. A biological material obtainable by the method and a therapeutic method for transplanting the biological material into a subject are also provided. An undifferentiated cell, in particular, preferably an ES cell is cultured to produce an increased embryoid body-like and the embryoid body-like is cultured for at least one week on, and adhered to, a three-dimensional solid type-I collagen carrier or an analogue thereof. Furthermore, a biological material is obtained thereby and the biological material is transplanted into a mammalian subject.Type: ApplicationFiled: August 27, 2007Publication date: January 21, 2010Inventors: Mariko Yamaki, Hidehiro Ozawa, Satoshi Ebina, Makoto Asashima
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Publication number: 20090291492Abstract: A method for forming an organ and/or tissue from undifferentiated cells derived from a vertebrate animal in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid X receptor ligand (e.g., a retinoic acid X receptor agonist or antagonist), and a method for forming a pancreas from undifferentiated cells derived from a vertebrate animal in vitro or a method for forming a tissue having morphology and function of a pancreas from undifferentiated cells derived from a vertebrate in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid receptor ligand, together with activin, that does not substantially bind to the retinoic acid receptor subtype ?.Type: ApplicationFiled: August 4, 2009Publication date: November 26, 2009Applicant: Research Foundation Itsuu LaboratoryInventors: Makoto ASASHIMA, Tatsuo HAMAZAKI, Hiroyuki KAGECHIKA, Koichi SHUDO
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Patent number: 7413901Abstract: The present invention provides a pancreas induced in vitro that is useful for developmental engineering and organ engineering, and a method wherein a pancreas induced in vitro which contributes to the development of diagnosis and treatment of pancreatic disorders for higher animals, can artificially and efficiently be induced from a gastrula apart from the presumptive region of pancreas. An explant which has a secretory gland-like structure wherein several cells are gathered and which expresses insulin is formed in vitro by treating the blastopore upper lip of an early gastrula of a vertebrate such as Xenopus with retinoic acid in vitro, and then culturing. The treatment with retinoic acid can be carried out, for example, by treating with retinoic acid at a concentration of 10?5 M or above for three hours.Type: GrantFiled: March 13, 2001Date of Patent: August 19, 2008Assignee: Japan Science and Technology AgencyInventors: Makoto Asashima, Naomi Moriya
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Publication number: 20080050817Abstract: The present invention discloses a medium for a serum-free medium capable of culturing ES cells for a long period while maintaining their undifferentiated state without using feeder cells, and a basal medium for producing the medium thus described. The basal medium of the present invention is characterized by that it has composition shown by Table I. Further, the present invention discloses a medium for ES cells produced with the basal medium.Type: ApplicationFiled: December 27, 2004Publication date: February 28, 2008Inventors: Miho Furue, Tetsuji Okamoto, Makoto Asashima
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Patent number: 7291500Abstract: In order to grow an object to be cultivated or grown, a growth object is encapsulated in a vessel, and the growth object is grown without substantial influence of gravity.Type: GrantFiled: May 18, 2005Date of Patent: November 6, 2007Assignees: Mitsubishi Heavy Industries, Ltd.Inventors: Masaru Uemura, Jun-ichiro Gyotoku, Makoto Asashima
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Publication number: 20070161105Abstract: A method for forming an organ and/or tissue from undifferentiated cells derived from a vertebrate animal in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid X receptor ligand (e.g., a retinoic acid X receptor agonist or antagonist), and a method for forming a pancreas from undifferentiated cells derived from a vertebrate animal in vitro or a method for forming a tissue having morphology and function of a pancreas from undifferentiated cells derived from a vertebrate in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid receptor ligand, together with activin, that does not substantially bind to the retinoic acid receptor subtype ?.Type: ApplicationFiled: March 17, 2004Publication date: July 12, 2007Applicant: REASEARCH FOUNDATION ITSUU LABORATORYInventors: Makoto Asashima, Tatsuo Hamazaki, Hiroyuki Kagechika, Koichi Shudo
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Patent number: 7112441Abstract: In order to grow an object to be cultivated or grown, a growth object is encapsulated in a vessel, and the growth object is grown without substantial influence of gravity.Type: GrantFiled: September 4, 2002Date of Patent: September 26, 2006Assignees: Mitsubishi Heavy Industries, Ltd.Inventors: Masaru Uemura, Jun-Ichiro Gyotoku, Makoto Asashima