Abstract: To detect a biological substance at high speed and high sensitivity by using a well array and particles. The biological substance detection method of the present invention includes a step of preparing a test liquid containing particles at a concentration of at least 5×107 particles/mL, a step of allowing the particles to trap the biological substance to form a trapped body, a step of sending the test liquid onto the well array to store a plurality of the particles including the trapped body in the wells while storing the number of the particles to be stored in one well to a minimum average storage number Nmin represented by the following formulas (1) and (2), and a step of detecting the color development of the well with an image pickup element.

Abstract: An object is to detect a virus with high speed and high sensitivity. Provided is a virus detection method including using a well array formed by defining a plurality of wells adjacent to each other by a side wall stood on a substrate and detecting a virus in a test liquid based on the detection of light emission from the wells, wherein the well array has a test region in which a well formation area, which is an area of the substrate in a region where the wells are formed, is 5 mm2 or more, a total volume of the wells obtained by integrating individual volumes of the wells is 17 nL or more, a depth of the wells is 3.5 ?m to 40 ?m, an opening area of the wells is 18 ?m2 to 1,700 ?m2, a volume of the wells is 74 fL to 6,000 fL, and a thickness of the side wall between the wells adjacent to each other is 0.5 ?m to 15 ?m; and the detection of light emission from the wells is performed in a detection unit in which an observation visual field is fixed to have a size including the test region.

Abstract: Provided is a target substance detection device that can detect a target substance accurately and efficiently and can be manufactured at low costs.

Abstract: A detection method including forming a complex by binding, to a target substance, a first substance immobilized to a metal particle with magnetism and a second substance labeled with a fluorescent material, moving the complex by applying a magnetic field, illuminating the complex during movement with excitation light of a predetermined wavelength, the excitation light causing the fluorescent material to emit fluorescence, the fluorescence being enhanced by localized surface plasmon resonance that is produced by the metal particle, capturing the enhanced fluorescence over time and obtaining two-dimensional images, and detecting the target substance in accordance with a light spot included in each of the two-dimensional images, the metal particle including an inner core made of a magnetic material and an outer shell covering the inner core, the outer shell being made of a nonmagnetic metal material that produces the localized surface plasmon resonance.

Abstract: To detect a target substance accurately and effectively, a target substance detection device 1 includes: a liquid-sample introducing plate 2 formed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unit 3 configured to be able to irradiate the liquid-sample introducing plate 2 with light from the rear face; a first magnetic field application unit 4 disposed on the side of the surface of the liquid-sample introducing plate 2, and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate 2; and an optical-signal detection unit 5 disposed on the side of the surface of th

Abstract: A target substance detection device detecting a target substance using magnetic particles includes a detection chip, a light irradiation unit, and a magnetic field application unit, wherein the detection chip includes a light transmissive member having a sensing surface arranged on a surface constituting a top surface relative to a bottom surface, an inclined surface, and a main body portion capable of receiving light and guiding the light through the interior, the light transmissive member having a light directing structure in which the light applied from the top surface side and passed through the inclined surface is directed via the main body portion to the sensing surface under the condition of total reflection, the light irradiation unit is operable to irradiate the sensing surface with light under the condition of total reflection via the light directing structure, and the magnetic field application unit.

Abstract: The invention relates to a method that detects the target substance by measuring a decrease in the optical signal generated by applying a first magnetic field to move the conjugate in the direction parallel to or away from the surface, etc. The invention also relates to an optical detection device wherein a liquid holding part has a sensing plate that can form a near field on a surface with light irradiated from a side of a rear face under a condition of total reflection, the liquid holding part being capable of holding a liquid sample that has a target substance and a magnetic particle that forms a conjugate with the target substance on the surface of the sensing plate, a light irradiation unit, a light detection unit, and a magnetic field application unit configured to apply a magnetic field to move the conjugate in the direction parallel to or away from the surface, etc.

Abstract: An optical detection method and an optical detection device quickly and accurately detects a micro target substance, such as an antigen, with high sensitivity by using an enhanced electric field. The optical detection device includes: one or more light irradiation units; a detection plate having a laminate structure; a prism in close optical contact to a back surface side of the detection plate and having multiple light incident surfaces with different incidence angles; and a light detection unit which is placed on the front surface side of the detection plate and which detects an optical signal from a sample. Light from the light irradiation unit enters the light incident surfaces of the prism at a fixed angle with respect to the front surface of the detection plate, and the light passing through the prism is irradiated from the back surface side of the detection plate under a total reflection condition.

Abstract: Provided is, among others, a target substance detection device that can be used in detecting a target substance using magnetic particles and that can be manufactured compactly and inexpensively.

Abstract: To detect a target substance accurately and effectively, a target substance detection device 1 includes: a liquid-sample introducing plate 2 formed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unit 3 configured to be able to irradiate the liquid-sample introducing plate 2 with light from the rear face; a first magnetic field application unit 4 disposed on the side of the surface of the liquid-sample introducing plate 2, and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate 2; and an optical-signal detection unit 5 disposed on the side of the surface of th

Abstract: Provided are an optical detection method and an optical detection device that quickly and accurately detect a micro target substance, such as an antigen, with high sensitivity by using an enhanced electric field.

Abstract: A problem is to provide an optical detection method that enables sensitive and quick detection of a micro target substance in a near field and such a device. As means for solving the problem, an optical detection method for a target substance detects light, such as fluorescence, as an optical signal from a conjugate comprising the target substance on a surface of a sensing plate in a near field, wherein the conjugate comprises at least the target substance bonding with a magnetic particle. The method comprises: detecting the target substance by measuring a decrease in the optical signal generated by applying a first magnetic field to move the conjugate in the direction parallel to or away from the surface, etc.

Abstract: [Problem] To provide a target substance detection chip, a target substance detection device, and a target substance detection method, that can be manufactured easily in a small size at low costs with reduction of the number of parts involved in the detection chip constituted by an optical prism and a detection plate used for a SPR sensor and an optical waveguide mode sensor, that can detect a target substance quickly with high sensitivity, and in which an analyte liquid is easily delivered.

Abstract: A detection device is disclosed which includes: a detection plate in which a silicon layer and a silicon oxide layer are arranged in this order on a silica glass substrate; and optical prism which is optically adhered to a surface of the silica glass substrate of the detection plate, where the surface is not provided with the silicon layer and the silicon oxide layer; a light-irradiation unit configured to irradiate light to the detection plate through the optical prism and arranged so that light is incident on the optical prism with a fixed incident angle; and a light-detection unit configured to detect intensity of reflected light reflected from the detection plate, wherein the detection device detects a change in dielectric constant by detecting a change in property of the reflected light.

Abstract: [Problem] To provide a target substance detection chip, a target substance detection device, and a target substance detection method, that can be manufactured easily in a small size at low costs with reduction of the number of parts involved in the detection chip constituted by an optical prism and a detection plate used for a SPR sensor and an optical waveguide mode sensor, that can detect a target substance quickly with high sensitivity, and in which an analyte liquid is easily delivered.

Abstract: [Problem] To provide a small-sized, stable, and highly sensitive detection device by achieving an optical system which is the most suitable for an optical waveguide mode sensor using a spectral measurement method.

Abstract: A chip is constituted from a transparent substrate, a reflector film formed on the transparent substrate and an optical waveguide layer formed on the reflector film, and a plurality of pores is formed in the optical waveguide layer. A light-introducing mechanism that introduces light from the transparent substrate of the chip onto the reflector film, and a light-detecting mechanism that detects the light reflected on the reflector film are provided. A specimen under investigation is detected by sensing a change in the incidence angle or in the intensity of reflected light that occurs when the specimen is adsorbed or deposits on the surface of the optical waveguide layer, by using a range of incidence angles of the light in which the intensity of reflected light changes when a part or all of the incident light couples with the optical waveguide mode that propagates in the optical waveguide layer.

Abstract: The present invention uses a detecting plate having a transparent substrate on which a single-crystal Si thin film layer, a transparent thin film layer, and a sample capturing layer for capturing a sample are provided. The present invention comprises a light directing mechanism for directing light through said transparent substrate of the detecting plate, and a light detection mechanism for detecting reflected light of the incident light from the detecting plate. The present invention is configured so that a change in absorbance occurs at the sample capturing layer or in the vicinity thereof, when said sample is captured on said sample capturing layer. The wavelengths of said incident light are determined in a range of wavelengths within which said change in absorbance occurs. In this way, the sample is detected by means of measuring a significant change in intensity of the reflected light which is generated when the sample is captured on the sample capturing layer.

Abstract: A chip is constituted from a transparent substrate, a reflector film formed on the transparent substrate and an optical waveguide layer formed on the reflector film, and a plurality of pores is formed in the optical waveguide layer. A light-introducing mechanism that introduces light from the transparent substrate of the chip onto the reflector film, and a light-detecting mechanism that detects the light reflected on the reflector film are provided. A specimen under investigation is detected by sensing a change in the incidence angle or in the intensity of reflected light that occurs when the specimen is adsorbed or deposits on the surface of the optical waveguide layer, by using a range of incidence angles of the light in which the intensity of reflected light changes when a part or all of the incident light couples with the optical waveguide mode that propagates in the optical waveguide layer.

Abstract: The present invention aims to provide a chip applied to a molecular sensing device which carries out Raman spectroscopic analysis utilizing Raman scattering enhancement due to plasmons, and that achieves higher sensitivity and stability of its sensing sensitivity and miniaturization, and to provide a molecular sensing device including the chip. As the chip for Raman scattering enhancement applied to the molecular sensing device using the Raman spectroscopic analysis, which has an excitation light source for Raman scattering, a chip for Raman scattering enhancement and a photodetector for observing the Raman scattering, the present invention employs a chip having a molecular detecting element in which a transparent protection material thin film 32 composed of a dielectric material thin film or semiconducting material thin film is formed on a thin film 31 containing the noble metal oxide, and utilizes the Raman scattering enhancement by the thin film containing the noble metal oxide.