Patents by Inventor Makoto Kawamukai
Makoto Kawamukai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8163525Abstract: The present disclosure describes a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism. The present disclosure also describes a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a cell of a host organism.Type: GrantFiled: June 13, 2008Date of Patent: April 24, 2012Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima
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Patent number: 7723569Abstract: A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.Type: GrantFiled: April 29, 2005Date of Patent: May 25, 2010Assignees: National Institute of Agrobiological Sciences, National University Corporation Shimane University, Tokyo University of ScienceInventors: Koichi Kadowaki, Sakiko Takahashi, Makoto Kawamukai, Hiroaki Shimada
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Publication number: 20090130727Abstract: The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism. The present invention also relates to a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a host microorganism.Type: ApplicationFiled: June 13, 2008Publication date: May 21, 2009Inventors: Hideyuki MATSUDA, Makoto KAWAMUKAI, Kazuyoshi YAJIMA
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Patent number: 7402413Abstract: The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism. The present invention relates to a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a host microorganism.Type: GrantFiled: May 10, 2002Date of Patent: July 22, 2008Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima
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Publication number: 20080064074Abstract: The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Rhodotorula. The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1, 3 or 5 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity.Type: ApplicationFiled: August 10, 2007Publication date: March 13, 2008Applicant: KANEKA CORPORATIONInventors: Hideyuki MATSUDA, Makoto KAWAMUKAI, Kazuyoshi YAJIMA, Yasuhiro IKENAKA
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Patent number: 7320883Abstract: The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Aspergillus and genus Leucosporidium. The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1 and 2 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity.Type: GrantFiled: December 27, 2001Date of Patent: January 22, 2008Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka
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Publication number: 20060010519Abstract: A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.Type: ApplicationFiled: April 29, 2005Publication date: January 12, 2006Inventors: Koichi Kadowaki, Sakiko Takahashi, Makoto Kawamukai, Hiroaki Shimada
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Publication number: 20040234975Abstract: The present invention provides a process for producing coenzymes Q10 efficiently in microorganisms by utilizing a coenzymes Q10 side chain synthesis gene from a fungal species belonging to the genus Bulleromyces. The present invention relates to a DNA comprising a base sequence shown under SEQ ID NO:1, a DNA having a base sequence derived from the base sequence shown under SEQ ID NO:1 by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity, or a DNA capable of hybridizing with a DNA comprising the base sequence shown under SEQ ID NO:1 under a stringent condition and encoding a protein having decaprenyl diphosphate synthase activity.Type: ApplicationFiled: April 6, 2004Publication date: November 25, 2004Inventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima
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Publication number: 20040157286Abstract: The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Aspergillus and genus Leucosporidium.Type: ApplicationFiled: January 23, 2004Publication date: August 12, 2004Inventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka
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Publication number: 20040137567Abstract: The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism.Type: ApplicationFiled: March 19, 2004Publication date: July 15, 2004Inventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima
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Patent number: 6762037Abstract: The present invention has for its object to isolate a gene coding for the enzyme synthesizing the coenzyme Q10 side chain from a fungal strain of the genus Saitoella and exploit it to advantage for the efficient microbial production of coenzyme Q10. The present invention provides; a DNA having the nucleotide sequence shown under SEQ ID NO:1 a DNA having a nucleotide sequence derived from the nucleotide sequence of SEQ ID NO:1 by the deletion, addition, insertion and/or substitution of one or a plurality of nucleotides and coding for a protein having decaprenyl diphosphate synthase activity a DNA which hybridizes with the DNA having the nucleotide sequence of SEQ ID NO:1 under a stringent condition and codes for a protein having decaprenyl diphosphate synthase activity.Type: GrantFiled: July 23, 2001Date of Patent: July 13, 2004Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka, Junzo Hasegawa, Satomi Takahashi
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Publication number: 20040067566Abstract: The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Rhodotorula.Type: ApplicationFiled: July 30, 2003Publication date: April 8, 2004Inventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka
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Patent number: 6461842Abstract: A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q10 was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as Escherichia coli and expressing therein, coenzyme Q10 can be effectively produced.Type: GrantFiled: October 10, 2000Date of Patent: October 8, 2002Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka, Kenichi Nishi, Junzo Hasegawa, Satomi Takahashi
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Patent number: 6103488Abstract: The present invention relates to production of ubiquinone-10 in E. coli by isolating and sequencing a structural gene dds1 of decaprenyl diphosphate synthase derived from photosynthetic bacteria such as Rhodobacter capsulatus., and expressing it in E. coli.Type: GrantFiled: January 21, 1998Date of Patent: August 15, 2000Assignee: Alpha Foods Co., Ltd.Inventors: Hideyuki Matsuda, Makoto Kawamukai, Tsuyoshi Nakagawa, Katsunori Tanaka