Patents by Inventor Makoto Ojika

Makoto Ojika has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for determining the structure of oligosaccharide linkages and automated instrumentation thereof
    Patent number: 5492836
    Abstract: The invention provides a method for determining the structure of a carbohydrate sample, comprising perbenzoylating a carbohydrate sample with a perbenzoylating agent to protect free hydroxyl groups in the carbohydrate sample; cleaving the glycosidic linkages of the perbenzoylated carbohydrate sample by contacting the carbohydrate sample with an amount of BrCH.sub.2 COBr/H.sub.2 O effective to cleave the carbohydrate sample; treating the resulting product with AgOAc and methanol or AgOTf/TMU and methanol to effect glycosidation; treating the resulting product with thiourea to remove bromoacetate groups; subjecting the resulting product to effect methoxycinnamoylation of free hydroxyl groups; separating the resulting benzoates with high-pressure liquid chromatography; performing mass, ultraviolet and circular dichroic spectroscopy on the separated benzoates; and comparing the spectra so obtained with reference spectra or calculated values to identify the structure of the carbohydrate.
    Type: Grant
    Filed: March 6, 1991
    Date of Patent: February 20, 1996
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Koji Nakanishi, Harold V. Meyers, William T. Wiesler, Makoto Ojika