Patents by Inventor Makram M. Girgis

Makram M. Girgis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Purification and recovery of lipoprotein cholesterol
    Patent number: 5409840
    Abstract: Disclosed is an improved process for the recovery of cholesterol rich fractions from mammalian serum or plasma. The process involves adsorbing the fraction on precipitated silica gel agglomerates which are then separated from the serum or plasma whereupon the adsorbed cholesterol rich fraction is eluted from the silica and recovered.
    Type: Grant
    Filed: October 12, 1993
    Date of Patent: April 25, 1995
    Assignee: Miles Inc.
    Inventors: Makram M. Girgis, Thomas M. McCall, Joseph G. Montalto
  • Cholesterol-rich fraction useful in culture media and process of producing same
    Patent number: 4762792
    Abstract: A process is provided for isolating and purifying a cholesterol-rich fraction from mammalian blood plasma or serum. The resulting cholesterol-rich fraction is useful as a growth medium ingredient.
    Type: Grant
    Filed: October 28, 1986
    Date of Patent: August 9, 1988
    Assignee: Miles Inc.
    Inventors: Makram M. Girgis, David E. Jackson, Gerald L. Kruse, Frank J. Mannuzza, Joseph G. Montalto
  • Detection of cell membrane antigens and corresponding antibodies
    Patent number: 4403042
    Abstract: A method for detecting weakly expressed cell membrane antigens or antibodies thereto which involves contacting cells to be tested with a lipoprotein substance substantially free of heteroagglutins and isoagglutins, containing cholesterol and phospholipid. The lipoprotein substance is obtained from animal plasma.
    Type: Grant
    Filed: August 19, 1982
    Date of Patent: September 6, 1983
    Assignee: Miles Laboratories, Inc.
    Inventors: Wayne M. Henry, Frank J. Mannuzza, Makram M. Girgis
  • Purification of lipoprotein cholesterol for use as a cholesterol reference material
    Patent number: 4290774
    Abstract: A method for the purification of lipoprotein cholesterol. The method involves the steps of adsorbing lipoprotein material on a silica adsorbant, eluting the adsorbed lipoprotein with water at a pH of about 10 to 11.5, adjusting the salt concentration to about less than 0.05M, heating the lipoprotein to about 50.degree. to 100.degree. C. for a period of about 5 minutes to 24 hours, adding an alkaline carbonate and alkaline earth salt to form a precipitate and removing the precipitate, adjusting the pH from about 6.5 to 9.0 and recovering purified lipoprotein cholesterol. Also a method for using the cholesterol as a cholesterol reference material.
    Type: Grant
    Filed: January 7, 1980
    Date of Patent: September 22, 1981
    Assignee: Miles Laboratories, Inc.
    Inventors: Makram M. Girgis, David E. Jackson, Frank J. Mannuzza