Patents by Inventor Makusu Tsutsui

Makusu Tsutsui has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210285911
    Abstract: Provided are a virus measuring method, a virus measuring device, a virus determining program, a stress determining method, and a stress determining device. A virus measuring method includes a contact step of bringing a liquid specimen containing a body fluid of a subject and an electrolytic solution into contact with each other via a through-hole portion formed in a separating wall, a current measuring step of applying a voltage to the liquid specimen and the electrolytic solution with respect to the through-hole portion and obtaining a waveform of an ionic current flowing through the through-hole portion, and a virus determining step of determining the kind of a virus contained in the body fluid on the basis of the waveform. In the virus determining step, the kind of the virus is determined by comparing the waveform with waveform information that corresponds to a known virus and is obtained beforehand.
    Type: Application
    Filed: July 18, 2019
    Publication date: September 16, 2021
    Applicant: OSAKA UNIVERSITY
    Inventors: Kohji NISHIDA, Noriyasu HASHIDA, Masateru TANIGUCHI, Makusu TSUTSUI
  • Publication number: 20210270762
    Abstract: It is an object to improve detection accuracy of an object as compared with prior arts. A flow passage (10) provided in a detection device (10) includes a substrate (1) and a covering member (2) provided at a position corresponding to the substrate (1). A covering member opening (HL2) of the covering member (2) is provided such that a substrate opening (HL1) of the substrate (1) is not covered with the covering member (2). The covering member (2) is arranged onto the substrate (1) such that a substrate capacitance and a covering member capacitance are connected in series. The covering member capacitance is lower than the substrate capacitance.
    Type: Application
    Filed: July 10, 2019
    Publication date: September 2, 2021
    Inventors: Makusu Tsutsui, Kazumichi Yokota, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, Tomoji Kawai
  • Patent number: 10876159
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: December 29, 2020
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Patent number: 10877021
    Abstract: A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: December 29, 2020
    Assignees: OSAKA UNIVERSITY, TOKYO INSTITUTE OF TECHNOLOGY, NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY
    Inventors: Makusu Tsutsui, Kazumichi Yokota, Masateru Taniguchi, Tomoji Kawai, Mina Okochi, Masayoshi Tanaka, Yoshinobu Baba, Noritada Kaji, Takao Yasui, Yuji Miyahara, Yukichi Horiguchi
  • Publication number: 20200251184
    Abstract: The present invention provides a classification analysis method, a classification analysis device, and a storage medium for classification analysis, which enable, with high accuracy, the classification analysis of particulate or molecular analytes. As a means for solving the problem, a data group of particle-passage detection signals is based which are detected by a nanopore device 8 in accordance with passage of subject particles through a through-hole 12. A feature value is obtained in advance which indicates the feature of the waveform of the pulse signals corresponding to the passage of the predetermined analyte and the feature value obtained in advance is set as the learning data for the machine learning. The feature value obtained from the pulse signals of said analyzed data is set as a variable and the classification analysis on the predetermined analytes in the analyzed data can be performed by executing a classification analysis program due to the machine learning.
    Type: Application
    Filed: December 12, 2017
    Publication date: August 6, 2020
    Applicant: Osaka University
    Inventors: Takashi WASHIO, Tomoji KAWAI, Masateru TANIGUCHI, Makusu TSUTSUI, Kazumichi YOKOTA, Akira ISHI, Takeshi YOSHIDA
  • Publication number: 20200070169
    Abstract: Provided is a channel device that is capable of increasing the concentration of fine particles in a liquid only by use of fluid-dynamic flows without relying on electrostatic interactions. A channel device (1) in accordance with an embodiment of the present invention includes: a main channel (11) configured to allow a liquid containing fine particles to flow therethrough; a chamber (15) that is provided at an end of the main channel (11) and that is configured to store target fine particles which have increased in concentration; and a side channel (12) that is connected to a side face of the main channel (11) and that is configured to allow unwanted liquid to drain therethrough, wherein at least one of a height and a width of the side channel (12) is smaller than a particle size of the fine particles.
    Type: Application
    Filed: May 9, 2018
    Publication date: March 5, 2020
    Inventors: Wataru TONOMURA, Makusu TSUTSUI, Kazumichi YOKOTA, Akihide ARIMA, Masateru TANIGUCHI, Tomoji KAWAI
  • Publication number: 20190367979
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Application
    Filed: December 28, 2018
    Publication date: December 5, 2019
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Publication number: 20190257787
    Abstract: The present invention provides a number analyzing method, a number analyzing device, and a storage medium for number analysis, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device (8) in accordance with passage of subject particles through a through-hole (12). Also, a particle type distribution estimating program, which is a number deriving means, is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.
    Type: Application
    Filed: December 19, 2016
    Publication date: August 22, 2019
    Applicant: Osaka University
    Inventors: Takashi WASHIO, Tomoji KAWAI, Masateru TANIGUCHI, Makusu TSUTSUI, Kazumichi YOKOTA, Akira ISHI, Takeshi YOSHIDA
  • Publication number: 20190242846
    Abstract: Methods and systems are provided for creation of stable and consistent nanoelectrode pairs for detection of biomolecules, such as deoxyribonucleic acid.
    Type: Application
    Filed: February 4, 2019
    Publication date: August 8, 2019
    Inventors: Masateru TANIGUCHI, Takahito Ohshiro, Makusu Tsutsui, Kazumichi Yokota, Toshiki Matsuoka, Mark Oldham, Eric Nordman
  • Publication number: 20190128888
    Abstract: A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.
    Type: Application
    Filed: April 21, 2017
    Publication date: May 2, 2019
    Inventors: Makusu TSUTSUI, Kazumichi YOKOTA, Masateru TANIGUCHI, Tomoji KAWAI, Mina OKOCHI, Masayoshi TANAKA, Yoshinobu BABA, Noritada KAJI, Takao YASUI, Yuji MIYAHARA, Yukichi HORIGUCHI
  • Patent number: 10202644
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: February 12, 2019
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Patent number: 9726636
    Abstract: According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t<a <d?100a or (b) s<L, s<d?100s, t<L and t<d, wherein a, L and s are the diameter, length and width of the target, d is the diameter of the pore, and t is the thickness of the membrane in the proximity to the pore.
    Type: Grant
    Filed: September 12, 2014
    Date of Patent: August 8, 2017
    Assignees: KABUSHIKI KAISHA TOSHIBA, OSAKA UNIVERSITY
    Inventors: Sadato Hongo, Tomoji Kawai, Makusu Tsutsui, Masateru Taniguchi, Soh Ryuzaki
  • Publication number: 20160138101
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Application
    Filed: October 14, 2015
    Publication date: May 19, 2016
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Patent number: 9194838
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Grant
    Filed: March 1, 2011
    Date of Patent: November 24, 2015
    Assignee: OSAKA UNIVERSITY
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Publication number: 20140374255
    Abstract: According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t<a <d?100a or (b) s<L, s<d?100s, t<L and t<d, wherein a, L and s are the diameter, length and width of the target, d is the diameter of the pore, and t is the thickness of the membrane in the proximity to the pore.
    Type: Application
    Filed: September 12, 2014
    Publication date: December 25, 2014
    Applicants: KABUSHIKI KAISHA TOSHIBA, OSAKA UNIVERSITY
    Inventors: Sadato HONGO, Tomoji KAWAI, Makusu TSUTSUI, Masateru TANIGUCHI, Soh RYUZAKI
  • Publication number: 20140300339
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Application
    Filed: March 1, 2011
    Publication date: October 9, 2014
    Applicant: OSAKA UNIVERSITY
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Publication number: 20140055150
    Abstract: A maximum current value and pulse continuation duration are measured for each of plural pulses of tunnel current arising as a polynucleotide passes through between an electrode pair, and the polynucleotide base sequence is determined based on the maximum current value and the pulse continuation duration.
    Type: Application
    Filed: March 29, 2013
    Publication date: February 27, 2014
    Applicant: OSAKA UNIVERSITY
    Inventors: Tomoji Kawai, Takahito Ohshiro, Kazuki Matsubara, Masayuki Furuhashi, Makusu Tsutsui, Masateru Taniguchi
  • Publication number: 20140031995
    Abstract: Provided are: a control method and control device for the movement speed of a substance which are capable of controlling the movement speed of the substance with good precision, and of raising the durability of the device; and a use therefor. An substance with charge is caused to move by a movement path formed by a first electrical field and a second electrical field that are formed in directions that intersect with each other.
    Type: Application
    Filed: January 29, 2013
    Publication date: January 30, 2014
    Applicant: OSAKA UNIVERSITY
    Inventors: Tomoji Kawai, Makusu Tsutsui, Masateru Taniguchi