Patents by Inventor Makusu Tsutsui
Makusu Tsutsui has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240133790Abstract: An object is to provide a device that can measure a moving time (velocity) of a single particle with high accuracy, and an ion current measuring apparatus and a zeta potential measuring apparatus with the device, and an ion current measuring method and a zeta potential measuring method. The object can be achieved by a device used for measurement of ion current, the device including: a substrate; and a channel formed in the substrate. The channel includes a sample liquid supply channel, a sample collection channel, and constricted channel formed between the sample liquid supply channel and the sample collection channel. The constricted channel includes three or more constricted parts each formed with a protrusion part, the three or more constricted parts are formed substantially straight in a direction from the sample liquid supply channel to the sample collection channel, and when the width of each of the constricted parts is defined as 1, the spacing between adjacent constricted parts is 0.5 to 3.Type: ApplicationFiled: February 8, 2022Publication date: April 25, 2024Inventors: Makusu TSUTSUI, Shohei KISHIMOTO, Masateru TANIGUCHI
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Patent number: 11781099Abstract: A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.Type: GrantFiled: January 19, 2023Date of Patent: October 10, 2023Assignee: AIPORE INC.Inventors: Takashi Washio, Tomoji Kawai, Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Akira Ishi, Takeshi Yoshida
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Publication number: 20230159870Abstract: A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.Type: ApplicationFiled: January 19, 2023Publication date: May 25, 2023Applicant: AIPORE INC.Inventors: Takashi WASHIO, Tomoji KAWAI, Masateru TANIGUCHI, Makusu TSUTSUI, Kazumichi YOKOTA, Akira ISHI, Takeshi YOSHIDA
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Patent number: 11597898Abstract: A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.Type: GrantFiled: December 19, 2016Date of Patent: March 7, 2023Assignee: AIPORE INC.Inventors: Takashi Washio, Tomoji Kawai, Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Akira Ishii, Takeshi Yoshida
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Patent number: 11524295Abstract: Provided is a channel device that is capable of increasing the concentration of fine particles in a liquid only by use of fluid-dynamic flows without relying on electrostatic interactions. A channel device (1) in accordance with an embodiment of the present invention includes: a main channel (11) configured to allow a liquid containing fine particles to flow therethrough; a chamber (15) that is provided at an end of the main channel (11) and that is configured to store target fine particles which have increased in concentration; and a side channel (12) that is connected to a side face of the main channel (11) and that is configured to allow unwanted liquid to drain therethrough, wherein at least one of a height and a width of the side channel (12) is smaller than a particle size of the fine particles.Type: GrantFiled: May 9, 2018Date of Patent: December 13, 2022Assignee: AIPORE INC.Inventors: Wataru Tonomura, Makusu Tsutsui, Kazumichi Yokota, Akihide Arima, Masateru Taniguchi, Tomoji Kawai
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Patent number: 11307161Abstract: It is an object to improve detection accuracy of an object as compared with prior arts. A flow passage (10) provided in a detection device (10) includes a substrate (1) and a covering member (2) provided at a position corresponding to the substrate (1). A covering member opening (HL2) of the covering member (2) is provided such that a substrate opening (HL1) of the substrate (1) is not covered with the covering member (2). The covering member (2) is arranged onto the substrate (1) such that a substrate capacitance and a covering member capacitance are connected in series. The covering member capacitance is lower than the substrate capacitance.Type: GrantFiled: July 10, 2019Date of Patent: April 19, 2022Assignee: Aipore Inc.Inventors: Makusu Tsutsui, Kazumichi Yokota, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, Tomoji Kawai
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Publication number: 20210285911Abstract: Provided are a virus measuring method, a virus measuring device, a virus determining program, a stress determining method, and a stress determining device. A virus measuring method includes a contact step of bringing a liquid specimen containing a body fluid of a subject and an electrolytic solution into contact with each other via a through-hole portion formed in a separating wall, a current measuring step of applying a voltage to the liquid specimen and the electrolytic solution with respect to the through-hole portion and obtaining a waveform of an ionic current flowing through the through-hole portion, and a virus determining step of determining the kind of a virus contained in the body fluid on the basis of the waveform. In the virus determining step, the kind of the virus is determined by comparing the waveform with waveform information that corresponds to a known virus and is obtained beforehand.Type: ApplicationFiled: July 18, 2019Publication date: September 16, 2021Applicant: OSAKA UNIVERSITYInventors: Kohji NISHIDA, Noriyasu HASHIDA, Masateru TANIGUCHI, Makusu TSUTSUI
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Publication number: 20210270762Abstract: It is an object to improve detection accuracy of an object as compared with prior arts. A flow passage (10) provided in a detection device (10) includes a substrate (1) and a covering member (2) provided at a position corresponding to the substrate (1). A covering member opening (HL2) of the covering member (2) is provided such that a substrate opening (HL1) of the substrate (1) is not covered with the covering member (2). The covering member (2) is arranged onto the substrate (1) such that a substrate capacitance and a covering member capacitance are connected in series. The covering member capacitance is lower than the substrate capacitance.Type: ApplicationFiled: July 10, 2019Publication date: September 2, 2021Inventors: Makusu Tsutsui, Kazumichi Yokota, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, Tomoji Kawai
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Patent number: 10877021Abstract: A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.Type: GrantFiled: April 21, 2017Date of Patent: December 29, 2020Assignees: OSAKA UNIVERSITY, TOKYO INSTITUTE OF TECHNOLOGY, NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITYInventors: Makusu Tsutsui, Kazumichi Yokota, Masateru Taniguchi, Tomoji Kawai, Mina Okochi, Masayoshi Tanaka, Yoshinobu Baba, Noritada Kaji, Takao Yasui, Yuji Miyahara, Yukichi Horiguchi
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Patent number: 10876159Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.Type: GrantFiled: December 28, 2018Date of Patent: December 29, 2020Assignee: QUANTUM BIOSYSTEMS INC.Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
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Publication number: 20200251184Abstract: The present invention provides a classification analysis method, a classification analysis device, and a storage medium for classification analysis, which enable, with high accuracy, the classification analysis of particulate or molecular analytes. As a means for solving the problem, a data group of particle-passage detection signals is based which are detected by a nanopore device 8 in accordance with passage of subject particles through a through-hole 12. A feature value is obtained in advance which indicates the feature of the waveform of the pulse signals corresponding to the passage of the predetermined analyte and the feature value obtained in advance is set as the learning data for the machine learning. The feature value obtained from the pulse signals of said analyzed data is set as a variable and the classification analysis on the predetermined analytes in the analyzed data can be performed by executing a classification analysis program due to the machine learning.Type: ApplicationFiled: December 12, 2017Publication date: August 6, 2020Applicant: Osaka UniversityInventors: Takashi WASHIO, Tomoji KAWAI, Masateru TANIGUCHI, Makusu TSUTSUI, Kazumichi YOKOTA, Akira ISHI, Takeshi YOSHIDA
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Publication number: 20200070169Abstract: Provided is a channel device that is capable of increasing the concentration of fine particles in a liquid only by use of fluid-dynamic flows without relying on electrostatic interactions. A channel device (1) in accordance with an embodiment of the present invention includes: a main channel (11) configured to allow a liquid containing fine particles to flow therethrough; a chamber (15) that is provided at an end of the main channel (11) and that is configured to store target fine particles which have increased in concentration; and a side channel (12) that is connected to a side face of the main channel (11) and that is configured to allow unwanted liquid to drain therethrough, wherein at least one of a height and a width of the side channel (12) is smaller than a particle size of the fine particles.Type: ApplicationFiled: May 9, 2018Publication date: March 5, 2020Inventors: Wataru TONOMURA, Makusu TSUTSUI, Kazumichi YOKOTA, Akihide ARIMA, Masateru TANIGUCHI, Tomoji KAWAI
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Publication number: 20190367979Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.Type: ApplicationFiled: December 28, 2018Publication date: December 5, 2019Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
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Publication number: 20190257787Abstract: The present invention provides a number analyzing method, a number analyzing device, and a storage medium for number analysis, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device (8) in accordance with passage of subject particles through a through-hole (12). Also, a particle type distribution estimating program, which is a number deriving means, is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.Type: ApplicationFiled: December 19, 2016Publication date: August 22, 2019Applicant: Osaka UniversityInventors: Takashi WASHIO, Tomoji KAWAI, Masateru TANIGUCHI, Makusu TSUTSUI, Kazumichi YOKOTA, Akira ISHI, Takeshi YOSHIDA
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Publication number: 20190242846Abstract: Methods and systems are provided for creation of stable and consistent nanoelectrode pairs for detection of biomolecules, such as deoxyribonucleic acid.Type: ApplicationFiled: February 4, 2019Publication date: August 8, 2019Inventors: Masateru TANIGUCHI, Takahito Ohshiro, Makusu Tsutsui, Kazumichi Yokota, Toshiki Matsuoka, Mark Oldham, Eric Nordman
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Publication number: 20190128888Abstract: A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.Type: ApplicationFiled: April 21, 2017Publication date: May 2, 2019Inventors: Makusu TSUTSUI, Kazumichi YOKOTA, Masateru TANIGUCHI, Tomoji KAWAI, Mina OKOCHI, Masayoshi TANAKA, Yoshinobu BABA, Noritada KAJI, Takao YASUI, Yuji MIYAHARA, Yukichi HORIGUCHI
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Patent number: 10202644Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.Type: GrantFiled: October 14, 2015Date of Patent: February 12, 2019Assignee: QUANTUM BIOSYSTEMS INC.Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
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Patent number: 9726636Abstract: According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t<a <d?100a or (b) s<L, s<d?100s, t<L and t<d, wherein a, L and s are the diameter, length and width of the target, d is the diameter of the pore, and t is the thickness of the membrane in the proximity to the pore.Type: GrantFiled: September 12, 2014Date of Patent: August 8, 2017Assignees: KABUSHIKI KAISHA TOSHIBA, OSAKA UNIVERSITYInventors: Sadato Hongo, Tomoji Kawai, Makusu Tsutsui, Masateru Taniguchi, Soh Ryuzaki
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Publication number: 20160138101Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.Type: ApplicationFiled: October 14, 2015Publication date: May 19, 2016Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
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Patent number: 9194838Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.Type: GrantFiled: March 1, 2011Date of Patent: November 24, 2015Assignee: OSAKA UNIVERSITYInventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai