Abstract: The present invention provides compositions, combinations, methods, sequences and kits for use of novel fluorescent proteins derived from the genus Branchiostoma. Specifically, polynucleotide and polypeptide sequences encoding fluorescent proteins isolated from Branchiostoma floridae, including harmonized sequences, which permit enhanced expression of the encoded polypeptides in mammalian cells in vivo are provided.

Abstract: The present invention provides compositions, combinations, methods, sequences and kits for use of novel fluorescent proteins derived from the genus Branchiostoma. Specifically, polynucleotide and polypeptide sequences encoding fluorescent proteins isolated from Branchiostoma floridae, including harmonized sequences, which permit enhanced expression of the encoded polypeptides in mammalian cells in vivo are provided.

Abstract: A rotor (1) for use in a comminuting apparatus (100) is described and claimed. The rotor (1) includes a hub (2) rotatable about a first axis (A) and one or more disc members (4) for comminuting material. The disc members (4) are secured to the hub (2) and rotatable about a second axis (B), removed from the first axis (A). Drive means is provided to simultaneously rotate the hub (2) disc members (4) about their respective axes. Guides (11) may be provided below the disc members (4) to remove comminuted material after it has passed through a screen (10). The screen (10) may include base portion (10A) and side wall portion (10B), the side wall portion (10B) extending up to a level approximately level with the disc members (4). The guides (11) may include arms (11A) extending up beside the side wall portion (10B). A comminuting apparatus (100) including a rotor (1) is also claimed.