Abstract: Isolated nucleic acid molecules that include a minimally-sized, functional (minimally-functional) piggyBac transposon can incorporate (i) a 5? internal domain (ID) comprising a nucleotide fragment that is substantially homologous to a native piggyBac transposon sequence; (ii) a 5? terminal repeat domain (TRD) comprising a 5? terminal repeat (TR) sequence, a 5? spacer sequence, and a 5? internal repeat (IR) sequence; (ii) a sequence of interest; (iv) a 3? TRD comprising a 3? IR sequence, a 3? spacer sequence, and a 3? TR sequence; and (iv) a 3? ID comprising a nucleotide fragment that is substantially homologous to the native piggyBac transposon sequence. The 5? TRD and the 3? TRD can be optionally linked by a sequence comprising a multiple cloning site.

Abstract: Systems and methods for determining semantic place names from one or more location reports received from a user device are provided. High quality visits for a candidate semantic place location from a plurality of previously obtained location reports can be aggregated and used to generate a point cloud for the semantic place location. A high quality visit can correspond to a visit by a device that is determined to be associated with a candidate semantic place location with greater likelihood relative to a plurality of other candidate semantic place locations. Data associated with one or more point clouds can be accessed and used to support determinations of semantic place name for one or more location reports. In example embodiments, the semantic place name can be stored as part of a location history and/or provided for display in a user interface presented on a display device.

Abstract: Systems and methods for determining semantic place names from one or more location reports received from a user device are provided. High quality visits for a candidate semantic place location from a plurality of previously obtained location reports can be aggregated and used to generate a point cloud for the semantic place location. A high quality visit can correspond to a visit by a device that is determined to be associated with a candidate semantic place location with greater likelihood relative to a plurality of other candidate semantic place locations. Data associated with one or more point clouds can be accessed and used to support determinations of semantic place name for one or more location reports. In example embodiments, the semantic place name can be stored as part of a location history and/or provided for display in a user interface presented on a display device.

Abstract: The present invention provides a method for transforming an insect genome that has a much enhanced transformation frequency. The vectors and plasmids employed in the method are further described as transposition vectors that include a minimal amount of nucleotide sequence homologous to a 5? region and a 3? region of a native piggyBac nucleic acid sequence. The transformed cells or embryos may also be developed into transgenic organisms. Disclosed are minimal piggyBac-based plasmid constructs that comprises a minimal nucleic acid sequence homologous to a 5? end of a piggyBac nucleic acid sequence (about 60-80 bp, particularly 66 bp) and a relatively long (300 to about 380 bp, particularly 311 by or 378 bp) continuous nucleic acid sequence homologous to a 3? end of a piggyBac native nucleic acid sequence. Methods employing these constructs include the use of a helper plasmid.

Abstract: The piggyBac transposon is disclosed herein as an extremely versatile helper-dependent vector for gene transfer and germ line transformation in a wide range of vertebrate species. Presented are methods wherein genome sequencing databases may be examined using piggyBac, as homologues of piggyBac have been found among several sequenced animal genomes, including the human genome. This transposon is demonstrated to provide transposition in primate cells and embryos of the zebra fish, Danio rerio. PiggyBac mobility is demonstrated using an interplasmid transposition assay that consistently predicts the germ line transformation capabilities of this mobile element in several species. Both transfected COS-7 primate cells and injected zebrafish embryos supported the helper-dependent movement of tagged piggyBac element between plasmids in a cut-and-paste, TTAA target-site specific manner. The present invention discloses the use of piggyBac as a tool for genetic analysis of vertebrates.

Abstract: This invention relates, e.g., to transgenic insects, or progeny thereof, whose cells contain at least one genomically integrated, expressible, nucleic acid encoding two or more of a set of Nglycosylation enzymes that can glycosylate a heterologous protein with a mammalianized (e.g., humanized) glycosylation pattern. The glycosylation genes are preferably expressed in the insect cells in catalytic amounts. Also described are methods to use such a transgenic insect to produce heterologous, mammalianized polypeptides of interest.