Abstract: The present invention relates to a process for liquefying starch-containing material, comprising treating the starch-containing material with a bacterial alpha-amylase at a temperature between 70-90° C. for 10-120 minutes and a pullulanase at a temperature in the range from 40-60° C. for between 20 and 90 minutes. The invention also relates to a saccharification process for saccharifying liquefied starch-containing material, comprising saccharifying a liquefied starch-containing material in the presence of a carbohydrate-source generating enzyme and a pullulanase. Finally, the invention also relates to a process of producing a fermentation product, such as ethanol, comprising a liquefaction step and/or saccharification step carried out in accordance with the present invention.

Abstract: The present invention relates to a process of liquefying starch-containing material, with a bacterial alpha-amylase at a temperature in the range from 65-75° C. for 1 to 2 hours. The invention also relates to a process of producing a fermentation product, preferably ethanol, using the liquefaction process of the invention.

Abstract: The present invention relates to method of liquefying starch-containing material, wherein the method comprises the steps of (a) treating the starch-containing material with a bacterial alpha-amylase at a temperature around 70-90° C. for 15-90 minutes, and (b) treating the material obtained in step (a) with an alpha-amylase at a temperature between 60-80° C. for 30-90 minutes. The invention also relates to a process of producing a fermentation product, preferably ethanol, comprising a liquefaction step carried out according to the liquefaction method of the invention.