Abstract: Method for determining a level of activity of a biological pathway from a biological sample of a patient suffering from a pathology, the method comprising the steps of: measuring the level of expression of at least three genes from a group of individuals, control individuals or patients, for which the biological pathway is inactive, the at least three genes being associated to the biological pathway, for establishing a negative reference, and measuring level of expression of the at least three genes from a group of individuals, control individuals or patients, for which the biological pathway is active, for establishing a positive reference, and measuring the level of expression of the at least three genes of the patient in the sample.

Abstract: The present application concerns methods and compositions which can be used to detect cancer in mammals, in particular in humans. It notably describes serum markers of cancers and their uses in diagnosis methods. It also concerns tools and/or kits which can be used to implement these methods (reagents, probes, primers, antibodies, chips, cells, etc.), their preparation and their uses. The invention can be used to detect the presence or the progression of a cancer, particularly breast cancer, including at an early stage.

Abstract: The invention relates to a method for the in vitro diagnosis of breast cancer in a patient who may be suffering from a breast cancer, characterized in that it comprises the following steps: a) biological material is extracted from a biological sample taken from the patient, b) the biological material is brought into contact with at least 8 specific reagents chosen from the specific reagents for the target genes with a nucleic sequence having any one of SEQ ID Nos. 1 to 8, c) the expression of said target genes is determined.