Abstract: To provide a microstructure equipped with a mechanism for selectively detecting marker molecules expressed or secreted by individual cells forming a cell population, a method for fabricating such a microstructure, and specific solutions for detecting and identifying molecules to be detected using such a microstructure, the present invention provides a method for fabricating a hemispherical shell-shaped microstructure made of a thin film of a desired thickness and diameter, in which a material surface capable of fixing a probe for detecting a biomolecule is arranged on the inner surface, and a method for detecting a target biomolecule using such a thin film.

Abstract: A method of diagnosing a periodontal condition using a protein showing a concentration difference between periodontal tissue in a normal condition and an abnormal condition from a subject's saliva is provided. According to this non-invasive method, the patient himself is able to check the presence of periodontal disease and identify the time to receive a treatment at an early stage, which will allow the patient to save time and cost for the treatment of periodontitis.

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

Abstract: The present invention relates to a nucleic acid aptamer capable of binding to tebuconazole, mefenacet or inabenfide, and the use thereof, and more particularly, to a nucleic acid aptamer capable of binding specifically to a very small amount of tebuconazole, mefenacet or inabenfide, and a method of detecting and separating tebuconazole, mefenacet or inabenfide using the nucleic acid aptamer. The nucleic acid aptamer of the present invention, which can bind specifically to tebuconazole, mefenacet or inabenfide, enables the detection and separation of tebuconazole, mefenacet or inabenfide present in samples such as food in very small amounts, and thus is effective for the detection and removal of tebuconazole, mefenacet or inabenfide which was difficult to detect in the prior art.

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

Abstract: Disclosed herein is a recombinant vector for transforming a strain to detect benzoic acid and derivatives, comprising a bioluminescent gene encoding a bioluminescent protein and a gene set inducing the expression of the, bioluminescent gene wherein the gene set includes the regulatory gene nagR and a promoter region inducing the transcription of the bioluminescent gene via the action of protein NagR encoded by the gene nagR. Further disclosed are a transformant containing the recombinant vector and a method for detecting benzoic acid and derivatives, thereof by measuring bioluminescence generated after reacting the transformant with a sample to be tested.

Abstract: The present invention relates to recombinant bioluminescent bacteria Escherichia coli DH5@/pSodaLux (EBHJ1) [KCTC 10098BP] for monitoring oxidative stress. Recombinant vector pSodaLux including the SOD (superoxide dismutase) promoter gene is introduced to Escherichia coli to produce transformed recombinant bioluminescent bacteria Escherichia coli DH5@/pSodaLux (EBHJ1). These bacteria are sensitive in monitoring oxidative stress, which is fatal to living organisms.

Abstract: A bioreactor system of probing toxic materials using microorganisms comprises two reactors. A first-step reactor serves as a microorganism reservoir in which the microorganisms are continuously cultured in a constant phase and a second-step reactor, provided with fiber optics, offers a place in which the microorganisms meet the toxic materials for the first time. Since the microorganisms are so genetically recombinant as to luminesce upon reaction with toxic materials, light is generated in the second-step reactor and, then, transmitted along the fiber optics to a luminometer where the change in the intensity of the light is monitored over time. The microorganisms can be stably provided from the reservoir, so that it is possible to continuously monitor the light intensity and thus, to trace the pollution of the toxic materials. The toxic materials may come from any of the sources including rivers, waste water, sewage, agricultural and industrial water, household water, tap water, atomic power plants, etc.