Abstract: A method for tracking particles and life forms in three dimensions and in time. The present invention applies a numerical reconstruction approach to digital in-line holographic microscopy images in order to generate a time sequence hologram representing the trajectory of objects such as particles and life forms. By subtracting consecutive (in time) holograms of a particle sample volume and then adding the resulting differences, a final hologram is constructed that contains the time evolution of the object trajectory free from spurious background interference effects.

Abstract: A method for tracking particles and life forms in three dimensions and in time. The present invention applies a numerical reconstruction approach to digital in-line holographic microscopy images in order to generate a time sequence hologram representing the trajectory of objects such as particles and life forms. By subtracting consecutive (in time) holograms of a particle sample volume and then adding the resulting differences, a final hologram is constructed that contains the time evolution of the object trajectory free from spurious background interference effects.

Abstract: A novel scanning tunneling microscope (STM) is described which is constructed almost entirely of one metal e.g. aluminum and uses bimorph piezoelectric disks as the x, y, z drive elements. The design uses a simple, rugged tirpod configuration for the fine motion drive arms. Coarse motion of the sample, which is mounted on an aluminum holder, is achieved by pushing, or pulling, with a piezoelectric louse. Differential thermal expansion effects are avoided by design, to first order, and the resulting drift is .ltoreq.0.5 .ANG. per minute after only a short warm-up period. It is easy to build and operate, and has good immunity to mechanical vibrations.