Abstract: Disclosed herein are methods and devices for sectioning cell colonies. Also disclosed are methods of purifying cell colonies. A method of sectioning cell colonies can include providing a cell colony on a culture plate comprising a known thickness; positioning a bottom of the culture plate using automated focus technology; and sectioning the cell colony into one or more pieces using a pattern of laser cutting lines. Devices for performing the method are also disclosed.

Abstract: Disclosed herein are methods and devices for sectioning cell colonies. Also disclosed are methods of purifying cell colonies. A method of sectioning cell colonies can include providing a cell colony on a culture plate comprising a known thickness; positioning a bottom of the culture plate using automated focus technology; and sectioning the cell colony into one or more pieces using a pattern of laser cutting lines. Devices for performing the method are also disclosed.

Abstract: Some aspects relate to methods for genetic analysis of selected cells from within a heterogeneous population of cells. The population of cells first can be partitioned. Selected cells are identified by imaging, and then specifically targeted and lysed by irradiation with an energy beam, resulting in specific release of their cellular contents into the culture medium. The culture medium then can be sampled and assayed for the desired nucleic acids.

Abstract: Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.

Abstract: This invention provides a method and apparatus for selectively identifying, and targeting with an energy beam, specific cells within a cell population, for the purpose of inducing a response in the targeted cells. Using the present invention, every detectable cell in a population can be identified and affected, without substantially affecting non-targeted cells within the mixture.

Abstract: The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells.

Abstract: The invention relates to methods and apparatuses for introducing and releasing substances into and out of cells, and more specifically to methods and apparatuses for transiently permeabilizing a living cell so that any one or more of a variety of substances, such as ions, proteins, and nucleic acids, can be loaded into or released out of the cell.

Abstract: The invention provides an apparatus for electromagnetically affecting a particle of interest in a specimen. The apparatus includes (a) a stage capable of supporting the specimen; (b) a detector including at least one camera, wherein the detector is capable of resolving a particle of interest within the specimen; (c) a means for locating the particle of interest in three dimensions; (d) a means for focusing electromagnetic radiation to a focal volume within the specimen; and (e) a means for adjusting the relative positions of the stage and electromagnetic radiation focusing means, thereby positioning the particle of interest within the focal volume.

Abstract: The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells.

Abstract: The invention relates to methods and apparatuses for introducing and releasing substances into and out of cells, and more specifically to methods and apparatuses for transiently permeabilizing a living cell so that any one or more of a variety of substances, such as ions, proteins, and nucleic acids, can be loaded into or released out of the cell.

Abstract: The invention provides an apparatus for electromagnetically affecting a particle of interest in a specimen. The apparatus includes (a) a stage capable of supporting the specimen; (b) a detector including at least one camera, wherein the detector is capable of resolving a particle of interest within the specimen; (c) a means for locating the particle of interest in three dimensions; (d) a means for focusing electromagnetic radiation to a focal volume within the specimen; and (e) a means for adjusting the relative positions of the stage and electromagnetic radiation focusing means, thereby positioning the particle of interest within the focal volume.

Abstract: In a device (1) with a circuit (2) for generating a modulated signal (S) is provided a modulation device (9; 59) which is intended to receive a data signal (DS) and generate the modulated signal (S) as a function of the data signal (DS) where for the purpose of generating the modulated signal (S) as a function of the received data signal (DS) a choice can be made between at least two characteristic signal values (F1, F2; A1, A2) of the modulated signal (S) and further an adjustment device (13) is provided which is designed to adjust programmably and as a function of the data signal (DS) at least one of the characteristic signal values (F1, F2; A1, A2).

Abstract: A data carrier processing system is provided for receiving an amplitude-modulated carrier signal. The system includes rectifiers and a voltage limiter that limits the d.c. supply voltage that is provided by the rectifiers. The voltage limiter provides a delayed response to amplitude variations in the carrier signal, so as to avoid adverse effects on the modulation of the carrier signal, while also providing an un-delayed response to excess amplitude values, so as to avoid excessive d.c. supply voltage for circuit elements of the data carrier system.

Abstract: Two parallel regulator stages, each comprising a transistor (T1, T2, respectively), are provided. The transistor (T2) forms part of the indirect parallel regulator stage and acts directly on the rectifier. It is capable of taking up comparatively large currents and hence is comparatively slow. The transistor (T1) forms part of the direct regulator stage and operates in parallel with the sustaining capacitor. Because of the coarse control provided by the transistor (T2), it need take up small currents only and can hence have a very fast control behavior. Because of the coarse control by the transistor (T2), the alternating voltage on the antenna terminals (LA and LB) quickly breaks down when the RF signal is interrupted; consequently, the transmission rate can be increased in the case of pause interval modulation. Static discharges are also dissipated by the transistor (T2), so that no additional protective circuitry is required for this purpose.

Abstract: Process for emptying liquids and/or gases from a container, in particular from a shock absorber, in which a tool is used to penetrate the wall of the container to create a drain opening, whereby the tool is driven into the container by means of a pressure pulse. The pressure pulse is thereby generated by means of a controlled propellant charge inside a tool propellant chamber.

Abstract: In a shock absorber unit for vehicles of the double-tube type a piston rod guiding member is provided at respective first ends of a cylinder and a container surrounding the cylinder. The first end of the container engagingly surrounds a radially outer face of the piston rod guide member. The container is tube-shaped and has substantially constant wall thickness along its total length. The radially outer surface of the piston rod guiding member is provided with at least one radially inward directed recess or at least one radially outward directed projection. The first end of the container is deformed such as to engage this projection or recess respectively. A sleeve member engagingly surrounds the first end of the container. This sleeve member is also provided with a deformation which deformation is in engagement with the deformation of the container.

Abstract: In the illustrative embodiments described, a double tube shock absorber is provided with a continuously open throttling fluid passage device both on the piston valve unit and the bottom valve unit. The continuously open fluid passage device comprises a fluid passage chamber defined by a first wall member and a second wall member. The fluid passage chamber is connected to a first fluid chamber of the shock absorber by a first axially directed opening and to a second fluid chamber of the shock absorber by a second radially directed opening. The second radially directed opening is located radially outward of said first opening. The axial height of the fluid passage chamber continuously decreases in radial direction between said first opening and said second opening.

Abstract: A piston-cylinder unit for shock absorbers or the like is formed with a tubular container surrounding the cylinder and with a piston rod guide and sealing unit mounted at one end of the container with a support for the cylinder at the other end of the container. In order to effect a satisfactory sealing action at the fixing points of the cylinder, the cylinder is formed with a radial indentation.

Abstract: A fluid damped unit includes a cylinder, a piston rod arranged for extending and retracting movement from one end of the cylinder, the rod having a piston at its end within the cylinder, and a damper unit arranged in an annular work chamber formed between the piston rod and the cylinder for damping the movement of the piston rod. The damper unit includes a damping ring which is carried in a damping ring carrier, the ring frictionally contacting the inner circumferential surface of the cylinder. A stop ring is mounted to the piston rod for contacting the damping ring carrier when the piston rod is extended past a predetermind intermediate position toward a fully extended position wherein movement of the piston rod is hydraulically damped. The damping ring carrier is arranged to be fixed in the axial direction of the cylinder when the piston rod is retracted beyond its predetermined intermediate position.

Abstract: A hydro-pneumatic damping device particularly for spring legs of automotive vehicles comprises an inner cylinder, an outer container, a central cavity within the inner cylinder, an annular cavity between the inner cylinder and the outer container, a piston rod with a piston unit, venting passage means between the central cavity and the annular cavity extending across a cylinder closing unit and check valve means associated to said venting passage means. The check valve means comprise an annular check valve member in frictional sliding engagement with the piston rod for axial movement with the piston rod toward a sealing position during inward movement of the piston rod and toward an opening position during outward movement of the piston rod, first and second abutment means being provided for defining said sealing position and said opening position of said annular check valve member respectively.