Abstract: The present invention relates to a fast and reliable method for protected isothermal nucleic acids amplification (PINA) of DNA and RNA. Particularly, the invention relates to diagnostic methods for rapidly diagnosing, for example, an infectious agent, in a biological probe of interest. Particularly, the methods are suitable to be performed on a handheld and portable diagnostic system for performing the amplification method in a non-laboratory environment (“home-test”).

Abstract: The present invention relates to a fast and optionally multiplexing or multimeric method for isothermal amplification of nucleic acids, including DNA and RNA. Particularly, the invention relates to diagnostic methods for rapidly diagnosing, for example, at least two infectious agents, or at least two different targets in the same infectious agent, in a biological probe of interest. The invention further relates to a handheld and portable diagnostic system for performing the amplification method in a laboratory as well as in a non-laboratory environment. Further provided are suitable enzyme sequences, kits and uses of the method and the system.