Patents by Inventor Manfred Wiessler
Manfred Wiessler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9315537Abstract: In summary, the present invention concerns a method for multiple orthogonal labelling of oligonucleotides, preferably RNA or DNA, by simultaneously performing the inverse Diels-Alder reaction (DAinv) and the copper-catalyzed click reaction (CuAAC), wherein the method is employed in a single step by just adding the different reaction components together and incubating the aqueous reaction mixture preferably for one hour at room temperature. In detail, the reaction components are one or more N3-modified labels, a copper compound, a stabilizing ligand, a reducing agent and one or more electron-deficient label-modified dienes that are added together with an at least double-modified oligonucleotide having one more nucleotides containing one or more N3-reactive groups and one or more electron-rich dienophiles, wherein a terminal alkyne moiety is preferably used as N3-reactive group(s) and a frans-cyclooctene moiety or norbornene is preferably used as electron-rich dienophile(s), more preferably frans-cyclooctene.Type: GrantFiled: August 31, 2012Date of Patent: April 19, 2016Assignees: DEUTSCHES KREBSFORSCHUNGSZENTRUM, RUPRECHT-KARLS-UNIVERSITAT HEIDELBERGInventors: Juliane Becker, Andres Jäschke, Ayan Samanta, Manfred Wiessler
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Patent number: 9234120Abstract: The present invention concerns a method for applying a first metal onto a second metal, an isolator or semiconductor substrate by a Diels-Alder reaction, in particular a Diels-Alder reaction with inverse electron demand. The present invention further concerns the binding units L 1960 and F 160.Type: GrantFiled: August 12, 2011Date of Patent: January 12, 2016Assignees: DEUTSCHES KREBSFORSCHUNGSZENTRUM, FORSCHUNGSZENTRUM JUELICH GMBHInventors: Manfred Wiessler, Peter Lorenz, Heinz Fleischhacker, Karola Ursula Fleischhacker, Marlen Fleischhacker, Nadja Fleischhacker, Ranjita Ghosh-Moulick, Sandra Gilles, Dirk Mayer, Andreas Offenhaeusser
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Patent number: 9169283Abstract: The present invention concerns a method and a kit for the post-synthetic modification of nucleic acids via an inverse Diels-Alder reaction.Type: GrantFiled: July 25, 2012Date of Patent: October 27, 2015Assignees: DEUTSCHES KREBSFORSCHUNGSZENTRUM, RUPRECHT-KARLS-UNIVERSITAET HEIDELBERGInventors: Manfred Wiessler, Peter Lorenz, Heinz Fleischhacker, Karola Ursula Fleischhacker, Marlen Fleischhacker, Nadja Fleischhacker, Christian Kliem, Andres Jäschke, Juliane Schoch
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Publication number: 20150218203Abstract: In summary, the present invention concerns a method for multiple orthogonal labelling of oligonucleotides, preferably RNA or DNA, by simultaneously performing the inverse Diels-Alder reaction (DAinv) and the copper-catalyzed click reaction (CuAAC), wherein the method is employed in a single step by just adding the different reaction components together and incubating the aqueous reaction mixture preferably for one hour at room temperature. In detail, the reaction components are one or more N3-modified labels, a copper compound, a stabilizing ligand, a reducing agent and one or more electron-deficient label-modified dienes that are added together with an at least double-modified oligonucleotide having one more nucleotides containing one or more N3-reactive groups and one or more electron-rich dienophiles, wherein a terminal alkyne moiety is preferably used as N3-reactive group(s) and a frans-cyclooctene moiety or norbornene is preferably used as electron-rich dienophile(s), more preferably frans-cyclooctene.Type: ApplicationFiled: August 31, 2012Publication date: August 6, 2015Applicant: Deutsches KrebsforschungszentrumInventors: Juliane Becker, Andres Jäschke, Ayan Samanta, Manfred Wiessler
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Publication number: 20130274440Abstract: The present invention concerns a new dimedon derivative and a method for the purification of PNA and peptide oligomers.Type: ApplicationFiled: April 8, 2013Publication date: October 17, 2013Inventors: Anette JACOB, Jörg HOHEISEL, Marc DAUBER, Manfred WIESSLER, Peter LORENZ, Heinz FLEISCHHACKER, Hans-Christian KLIEM
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Patent number: 8552183Abstract: The present invention relates to a process for linking two molecules by means of a Diels Alder reaction with inverse electron requirement (DARinv), comprising the following steps: reaction of a (a) triazine or tetrazine with one or more electron-attracting substituents on the ring as a diene component, the electron-attracting substituents being selected from: COOR C(O)NR2 CX3 (X=halogen) halogen CN SO2—R or SO3—R PR2 wherein R?H, alkyl, aryl, heterocycle, which in turn may be substituted, where appropriate, with alkyl, OH, SH, halogen, aryl, heterocycle, nitro, carboxyamido or amine group.Type: GrantFiled: June 18, 2007Date of Patent: October 8, 2013Assignee: Deutsches Krebsforschungszentrum Stiftung des Offentlichen RechtsInventors: Manfred Wiessler, Eduard Müller, Peter Lorenz, Christian Kliem, Heinz Fleischhacker
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Publication number: 20130085271Abstract: The present invention concerns a method and a kit for the post-synthetic modification of nucleic acids via an inverse Diels-Alder reaction.Type: ApplicationFiled: July 25, 2012Publication date: April 4, 2013Inventors: Manfred WIESSLER, Peter Lorenz, Heinz Fleischhacker, Christian Kliem, Andres Jäschke, Juliane Schoch
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Publication number: 20110274618Abstract: The invention relates to the fields of materials sciences and medicine and relates to an agent, which can be used, for example, as a contrast medium for the localization of cancer cells. The object of the present invention is to disclose an agent which sensitively and selectively recognizes the site and the type of the molecules or cells to be examined. The object is attained through an agent composed at least of bio-shuttle molecules to which endohedral fullerenes are coupled by way of peptide-based molecules, wherein the endohedral fullerenes are hydrophobic and correspond to the formula A3-xMxZ@C2n in which x=0 to 3 and n?34, A means rare earths and/or transuranic elements, M means metals, Z means non-metals and C means carbon. The object is further attained through a method in which hydrophobic endohedral fullerenes are coupled with bio-shuttle molecules by way of an irreversible Diels-Alder reaction with an inverse electron demand (DARinv).Type: ApplicationFiled: November 10, 2009Publication date: November 10, 2011Applicant: Leibniz-Institut Fuer Festkoerper-Und Werkstoffforschung Dresden E.V.Inventors: Klaus Braun, Michael Bock, Ruediger Pipkorn, Waldemar Waldeck, Manfred Wiessler, Bernd Didinger, Juergen Debus, Volker Ehemann, Lothar Dunsch
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Publication number: 20100016545Abstract: The present invention relates to a process for linking two molecules by means of a Diels Alder reaction with inverse electron requirement (DARinv), comprising the following steps: reaction of a (a) triazine or tetrazine with one or more electron-attracting substituents on the ring as a diene component, the electron-attracting substituents being selected from: COOR C(O)NR2 CX3 (X=halogen) halogen CN SO2—R or SO3—R PR2 wherein R?H, alkyl, aryl, heterocycle, which in turn may be substituted, where appropriate, with alkyl, OH, SH, halogen, aryl, heterocycle, nitro, carboxyamido or amine group.Type: ApplicationFiled: June 18, 2007Publication date: January 21, 2010Applicant: DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES OFFENTLICHEN RECHTSInventors: Manfred Wiessler, Eduard Müller, Peter Lorenz, Christian Kliem, Heinz Fleischhacker
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Patent number: 6958395Abstract: The present invention relates to a method by which saccharide compounds can be prepared in a very easy way. This method comprises the steps of: (a) attaching at least one saccharide to a cyclic or acyclic diene, (b) reacting the saccharide-containing diene obtained in step (a) or a commercially available saccharide-containing diene with a dienophile by Diels-Alder reaction.Type: GrantFiled: August 22, 2001Date of Patent: October 25, 2005Assignee: Deutsches Krebsforschungszentrum Stiftung des Offentlichen RechtsInventors: Manfred Wiessler, Hans-Christian Kliem, Bernd Sauerbrei, Birgit Schmauser
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Patent number: 6743584Abstract: The invention relates to plasmids derived from pBluescript KS(+), which contains more than 1, preferably 2, 7, 14, 21 and 27, respective SK primer elements, and their use in analytical electron microscopy.Type: GrantFiled: March 1, 2002Date of Patent: June 1, 2004Assignee: Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen RechtsInventors: Sabine Bub, Helmut Troester, Karsten Richter, Ansgar Haking, Stefan Raddatz, Manfred Wiessler, Eberhard Spiess, Michael Trendelenburg
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Publication number: 20030153512Abstract: The invention relates to curcumin derivatives with improved water solubility compared to curcumin, which are characterized in that the curcumin part is linked to a monosaccharide, oligosaccharide or polysaccharide, and to medicaments containing these derivatives. The curcumin derivatives according to the invention are particularly suitable to prevent and treat cancer, chronic-inflammatory diseases and diseases associated with a retrovirus infection.Type: ApplicationFiled: April 8, 2003Publication date: August 14, 2003Inventors: Manfred Hergenhahn, Barbara Bertram, Manfred Wiessler, Bernda L Sorg
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Publication number: 20030119051Abstract: The present invention relates to a saccharide library with different saccharide-containing molecules, in which each of the molecules comprises a nuclear molecule with at least two functional groups and at least two saccharides. The invention also relates to the production of such a library and its use.Type: ApplicationFiled: July 29, 1999Publication date: June 26, 2003Inventors: MANFRED WIESSLER, CHRISTIAN KLIEM, WALTER MIER, STEFAN MENZLER
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Patent number: 6521604Abstract: The invention relates to a compound which contains boron and which comprises the following general formula (1) in which Cb represents carborane, R2 and R3, independent of one another, represent a hydrogen atom or an organic radical, and R and R1, independent of one another, represent a hydrogen atom or an organic group, or R and R1 form a carbonyl group with the carbon atom to which they are bound. The invention also relates to a method of producing a compound containing boron and to the application of the same.Type: GrantFiled: December 15, 2000Date of Patent: February 18, 2003Assignee: Deutches KrebsforschungszentrumInventors: Manfred Wiessler, Helmut Tröster, Stefan Raddatz, Eberhard Spiess, Michael Trendelenburg
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Patent number: 6489302Abstract: This invention relates to a conjugate, comprising a saccharide and one or more therapeutic or diagnostic agents, as well as the use of the conjugate. The conjugate of the present invention may be used for diagnosing cancer or viral diseases. The conjugate of the present invention may also be used to prevent and/or treat neurological diseases.Type: GrantFiled: April 20, 1998Date of Patent: December 3, 2002Assignee: Deutches Krebsforschungszentrum Stiftung Des Offentlichen RechtsInventors: Manfred Wiessler, Konrad Beyreuther
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Patent number: 6417339Abstract: The invention relates to dendrimers comprising an initiator core with at least two functional groups and at least two saccharides, a process for preparing sch dendrimers as well as uses thereof.Type: GrantFiled: March 8, 1999Date of Patent: July 9, 2002Assignee: Deutsches Krebsforschungzentrum Stiftung des Offentlichen RechtsInventors: Manfred Wiessler, Markus Gschrey, Willi Von der Lieth, Walter Mier
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Patent number: 6369109Abstract: The present invention is, generally, directed to the use of betulinic acid and derivatives thereof for the treatment of neuroectodermal tumors. The present invention is based on the discovery that betulinic acid and its derivatives are potent anti-neuroectodermal agents. As disclosed herein, betulinic acid and its derivatives are useful for the treatment of neuroectodermal tumors, including, due to its distinct mechanism of action, neuroectodermal tumors that are resistant to conventional chemotherapeutical agents. In addition to the new use of known compounds, the invention discloses novel compounds and pharmaceutical compositions for the treatment of neuroectodermal tumors.Type: GrantFiled: October 28, 1998Date of Patent: April 9, 2002Assignee: Deutsches Krebsforschungszentrum Stiftung des Offentlichen RechtsInventors: Klaus Michael Debatin, Simone Fulda, Manfred Wiessler, Marek Los, Walter Mier
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Patent number: 6211356Abstract: The invention concerns metallocene-phosphoramidite conjugates comprising one or a plurality of metallocenes and one or a plurality of phosphoramidites. The invention further concerns a process for preparing the metallocene-phosphoramidite conjugates and their use.Type: GrantFiled: October 21, 1998Date of Patent: April 3, 2001Assignee: Deutsches Krebsforschungszentrum Stiftung des Offentlichen RechtsInventors: Manfred Wiessler, Dagmar Schütte
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Patent number: 5622936Abstract: The invention relates to a method for the preparation of glycoconjugates of phosphorus amides with the general formula ##STR1## where the connection of the sugar with the phosphoric acid amide mustard residue, and the ifosfamide mustard residue, respectively, occurs preferably in the 1-position, and where R.sub.1 and R.sub.2 ; which can be the same or different, denote hydrogen, lower C.sub.1 -C.sub.4 alkyl or C.sub.1 -C.sub.6 haloalkyl and where as sugar there can be present mono-, di-, or polysaccharides in all existing isomeric and enantiomeric forms, wherein in a known way protected brominated sugars are conjugated with the respective phosphorus compounds, and freed of the protective residues, and to the use of said compounds as anti-tumour drugs.Type: GrantFiled: June 19, 1990Date of Patent: April 22, 1997Assignee: Deutsches Krebsforschungszentrum Stiftung des offentlichen RechtsInventors: Manfred Wiessler, Michael Dickes