Abstract: Devices for detecting microorganism strains or target cellular analytes are provided. The device includes a filter holder, the filter holder comprising a tip portion; a nonwoven article disposed on the tip portion of the filter holder; and an adaptor attacked to the filter holder, the adaptor defining an aperture. Methods of detecting microorganisms and/or cellular analytes in a fluid sample using the devices are also provided. The method includes obtaining the device; placing a lumened or cannulated device in fluid communication with the device; and passing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte from the lumened or cannulated device through the device and contacting the nonwoven article. The method further includes contacting the nonwoven article with at least one detection reagent and detecting the presence of the at least one microorganism strain or target cellular analyte concentrated by the nonwoven article.

Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to about 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.

Abstract: Fluid sensing devices including a layer of fluid absorption and distribution material are provided. The layer includes a porous fibrous nonwoven matrix for absorbing fluid from the object to the first major surface of the layer and laterally distributing the absorbed fluid throughout the layer and to a second major surface of the layer opposite the first major surface. Hydration sensors are disposed on the second major surface of the layer to measure a hydration level.

Abstract: Guanidine-functionalized particles and methods of making and using such particles are provided, such as guanidine-functionalized metal silicate particles. A nonwoven article is also provided including a porous fibrous nonwoven matrix and guanidine-functionalized metal silicate particles enmeshed within the porous fibrous nonwoven matrix. The nonwoven article may be used, for instance, for removing microorganisms from an aqueous sample by contacting a fluid sample with the nonwoven article such that at least a portion of the microorganisms are removed from the fluid sample.

Abstract: Porous devices are provided that include a particle-containing fibrous porous matrix and a fluid absorbed in the particle-containing porous fibrous nonwoven matrix. The particle-containing fibrous porous matrix includes a porous fibrous nonwoven matrix containing first polyolefin fibers, second polyolefin fibers including poly(ethylene), and fiberglass fibers; and microorganism-binding particles. The microorganism-binding particles are enmeshed in said porous fibrous nonwoven matrix. Methods of debridement are provided including providing a porous device including a particle-containing fibrous porous matrix and wiping a wound or an area of skin with the device; and providing a porous device including a porous fibrous nonwoven matrix and wiping a wound with the device. Further, kits are provided including a sterile package containing at least one porous device, and typically also instructions for debridement.

Abstract: Guanidine-functionalized perlite particles are provided. Nonwoven articles are also provided, including a fibrous porous matrix and guanidine-functionalized perlite particles enmeshed in the fibrous porous matrix. Laminated articles are additionally provided, including a first substrate and a second substrate sealed to the first substrate along at least a portion of a perimeter of the first substrate. The laminated article further includes guanidine-functionalized perlite particles disposed between the first substrate and the second substrate. Methods of detecting microorganisms or target cellular analytes in a fluid sample using guanidine-functionalized particles or laminated articles are also provided.

Abstract: Porous articles are provided that include a fibrous porous matrix and porous polymeric particles. The porous polymeric particles are distributed throughout the fibrous porous polymeric matrix. The porous article can be used to prepare a separation device or a system that includes the separation device. The porous articles can be used for the separation of a target material such as a microorganism (i.e., cellular analyte) from a sample.

Abstract: Laminated articles are provided including a porous fibrous nonwoven matrix and guanidine-functionalized metal silicate particles enmeshed within the porous fibrous nonwoven matrix. The laminated articles further include a first substrate and a second substrate sealed to the first substrate. Methods of making laminated articles and methods of using laminated articles are also provided.

Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.

Abstract: The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.

Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising ferric oxide, titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.

Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises a metal silicate, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte.

Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising ferric oxide, titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.

Abstract: A bismuth-containing concentration agent for microorganisms is provided. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.

Abstract: Nonwoven articles for detecting microorganisms or cellular analytes in a fluid sample are provided. The nonwoven article includes a fibrous porous matrix and concentration agent particles enmeshed in the fibrous porous matrix. The fibrous porous matrix generally consists of inorganic fibers and polymeric fibers. Methods of detecting microorganisms or cellular analytes in a fluid sample are also provided. The method includes providing the nonwoven article, providing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte, contacting the fluid sample with the nonwoven article such that at least a portion of the at least one microorganism strain or target cellular analyte is bound to the nonwoven article, and detecting the presence of bound microorganism strain(s) or bound cellular analyte(s).

Abstract: Devices for detecting microorganism strains or target cellular analytes are provided. The device includes a filter holder, the filter holder comprising a tip portion; a nonwoven article disposed on the tip portion of the filter holder; and an adaptor attacked to the filter holder, the adaptor defining an aperture. Methods of detecting microorganisms and/or cellular analytes in a fluid sample using the devices are also provided. The method includes obtaining the device; placing a lumened or cannulated device in fluid communication with the device; and passing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte from the lumened or cannulated device through the device and contacting the nonwoven article. The method further includes contacting the nonwoven article with at least one detection reagent and detecting the presence of the at least one microorganism strain or target cellular analyte concentrated by the nonwoven article.

Abstract: Methods of detecting microorganisms in a fluid sample are provided. The method includes providing a nonwoven article, providing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte, and contacting the fluid sample with the nonwoven article such that at least a portion of the at least one microorganism strain or target cellular analyte is bound to the nonwoven article. The nonwoven article includes a fibrous porous matrix and a concentration agent particles enmeshed in the fibrous porous matrix. The method further includes placing the microorganism strain- or target cellular analyte-bound nonwoven article in contact with at least one detection reagent and detecting the presence of the bound microorganism strain or bound target cellular analyte. Devices and kits containing the devices, for contacting a fluid sample with a nonwoven article, are also provided.

Abstract: Guanidine-functionalized perlite particles are provided. Nonwoven articles are also provided, including a fibrous porous matrix and guanidine-functionalized perlite particles enmeshed in the fibrous porous matrix. Laminated articles are additionally provided, including a first substrate and a second substrate sealed to the first substrate along at least a portion of a perimeter of the first substrate. The laminated article further includes guanidine-functionalized perlite particles disposed between the first substrate and the second substrate. Methods of detecting microorganisms or target cellular analytes in a fluid sample using guanidine-functionalized particles or laminated articles are also provided.

Abstract: Porous devices are provided that include a particle-containing fibrous porous matrix and a fluid absorbed in the particle-containing porous fibrous nonwoven matrix. The particle-containing fibrous porous matrix includes a porous fibrous nonwoven matrix containing first polyolefin fibers, second polyolefin fibers including poly(ethylene), and fiberglass fibers; and microorganism-binding particles. The microorganism-binding particles are enmeshed in said porous fibrous nonwoven matrix. Methods of debridement are provided including providing a porous device including a particle-containing fibrous porous matrix and wiping a wound or an area of skin with the device; and providing a porous device including a porous fibrous nonwoven matrix and wiping a wound with the device. Further, kits are provided including a sterile package containing at least one porous device, and typically also instructions for debridement.

Abstract: The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.