Abstract: The present invention relates to a needle having safety mechanism for a huber needle assembly in which the safety mechanism comprises a needle hub, a base, a huber needle slidably disposed through the needle hub, a wing holder having two wings at the opposite end of the wing holder, a connector which connects the needle hub and the base for protecting the tip of the huber needle.

Abstract: Manipulation of male fertility in a polyploid species requires attention to the interaction of male-fertility alleles of multiple genomes. In hexaploid wheat, single-genome heterozygotes for Ms26 provide differential levels of male fertility across genomes. Hexaploid wheat homozygous for mutations in the Ms26 gene on the A, B, and D genomes is male-sterile. Male fertility may be restored by sufficient levels of expression of Ms26 using native Ms26 or a transgene, which may be native to wheat or to another species, or a combination of native and transgenic alleles. CRISPR/Cas9 technology may be used to generate mutations in Ms26 in wheat or rice.

Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

Abstract: Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.

Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

Abstract: Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.

Abstract: A lock system is described which includes a range monitoring system including a computer processor to monitor the proximity of the bin to a base station; a collection monitoring module including a computer processor to monitor when the bin is being collected. Also described is a computer-controlled method of inhibiting bin access to unauthorised persons, the method includes the steps of monitoring proximity of a bin to a base station with a range monitor having a computer processor to monitor the proximity of the bin; monitoring bin collection movement with a computer processor having a lift monitor, and unlocking a bin lock with a computer controller when the range monitor detects that the bin is proximal a base station and locking the bin lock when the range monitor detects that the bin is distal the base station.

Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms1 or Ms5 nucleotide sequence to a male-sterile Ms1 or Ms5 plant.

Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms1 or Ms5 nucleotide sequence to a male-sterile Ms1 or Ms5 plant produced using the methods and compositions described herein.

Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.

Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.

Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably-linked gene. Mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations may be tracked. Male-sterile plants may be maintained by crossing with a maintainer plant. Hybrids can be produced using the male-sterile plants.

Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably-linked gene. Mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations may be tracked. Male-sterile plants may be maintained by crossing with a maintainer plant. Hybrids can be produced using the male-sterile plants.

Abstract: Compositions and methods are provided for screening wheat seed for sorting and selection. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which encode and express a screenable color marker in seeds. Expression cassettes comprise a plant-derived polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the color, opacity, fluorescence, or other property of the seed. The plant-derived marker can be used in a male-sterile production system of hybrid wheat seed. Methods for maintaining a line of male-sterile plants and for restoring male fertility in a male-sterile plant, comprising a screenable color marker are provided.

Abstract: The invention relates to an arrangement and a method performing data exchange between various integrated circuits, IC, (3,4,5,6,7) in an automotive control system wherein the data are exchanged by a bus and has the object to enable ASIL C/D system coverage and to tie various ICs (clocks, regulators, memory interfaces, sensor signal conditioners, power management ICs etc.) This is solved the data are exchanged by a bus being ASIL C/D compliant and forming a common protocol to exchange information among the integrated circuits (3,4,5,6,7). The method is solved by functions implemented within the bus as setting the frequency of operation; arbitrating roles of the integrated circuits as master or slave device; checking integrity of exchanged data; frame repetition; detecting bus stuck-at failure modes; filtering or denouncing failures and warnings from peripheral devices; detecting remote out of specification local clock; and monitoring and predicting system reliability and profiling maintenance events.

Abstract: The present invention relates to a needle having safety mechanism and particularly relates to a huber needle assembly (10; 110) having safety mechanism comprising a needle hub (12; 112), a base (14; 114), a huber needle (16; 116) slidably disposed through the needle hub (12; 112), a wing holder (16; 116) having two wings (18; 118) at the opposite end of the wing holder (16; 116), a connector (24; 124) which connect the needle hub (12; 112) and the base (14; 114) for protecting the tip of the huber needle.

Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.

Abstract: Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.

Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms1 or Ms5 nucleotide sequence to a male-sterile Ms1 or Ms5 plant produced using the methods and compositions described herein.