Abstract: Disclosed are construction and application of a recombinant H5N8 subtype avian influenza virus carrying an mApple fluorescence reporter gene, and relates to the field of molecular biology and genetic engineering. The present disclosure provides a nucleic acid molecule which can be used for constructing the recombinant H5N8 subtype avian influenza virus. Compared with a wild H5N8 virus, the recombinant H5N8 subtype avian influenza virus has a weakened virulence, and can replicate in an SPF chicken lung and shows pathogenicity.

Abstract: Disclosed are a restrictive epitope peptide of a major histocompatibility complex (MHC) B2 of an H9N2 subtype Avian influenza virus (AIV) and an application thereof, and relate to the technical field of genetic engineering. The restrictive epitope peptide has an amino acid sequence as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 or SEQ ID No.4.

Abstract: An identification and an application of an ALV-J MHC-B2 restrictive epitope peptide are provided, which belong to the field of genetic engineering. An amino acid sequence of the provided ALV-J MHC-B2 restrictive epitope peptide is selected from SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3. An application of the ALV-J MHC-B2 restrictive epitope peptide in preparing an ALV epitope-based vaccine is provided. Restrictive motif of B2-haplotype chicken MHC class I molecule binding peptides is identified by in vitro elution assay. Potential epitopes in four proteins expressed by ALV-J are systematically screened by motif. The immunogenic B2-haplotype chicken ALV-J T-cell epitope peptides are identified by functional validation. It provides a material and theoretical basis for the research and development of ALV epitope-based vaccines.