Patents by Inventor Manman ZHAO

Manman ZHAO has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210115437
    Abstract: The present invention provides an in vitro synthesis method for an sgRNA and a kit thereof. Specifically, the present invention provides a nucleic acid construct having a structure represented by formula I from 5? to 3?: Y1-L1-Y2-L2-Y3-Y4 (I), wherein Y1 is an RNA polymerase promoter region; L1 is absent or a linking sequence; Y2 is a target DNA sequence; L2 is absent or a linking sequence; Y3 is a downstream primer binding region; Y4 is absent or a nucleotide sequence; and each “-” is independently a bond or a nucleotide linking sequence.
    Type: Application
    Filed: March 29, 2019
    Publication date: April 22, 2021
    Inventors: Huaxing ZHU, Qingyi ZHANG, Jiajia SHI, Manman ZHAO, Yi HE