Abstract: Artificial intelligence systems and computer-implemented methods for generating digital twins, establishing wellness parameters and generating recommendations to improve performance and wellness. An artificial intelligence system comprises a biosample collection unit, a physical analysis unit to perform a detection, relative quantification and untargeted analysis of analytes from the biosamples, a computing unit comprising: an automatic data collection unit, a data analysis unit for analysing the data, and a real-time data computing integration unit which automatically integrates the data generated from the analysis, transforms the data to establish representing values of wellness parameters through time, compares the representing values with data stored in the automatic data collection unit and responds to a change by generating a set of recommended actions or patterns response to improve performance and wellness.

Abstract: Provided herein is a method of using digital twin biomarker profiles for improved sporting, therapy, wellness and healthcare outcomes by comparing digital twin biomarker profiles at discrete interval to discern and evaluate data progression. Also provided is a sampling kit for collecting human fluid samples for analysis and generation of digital twin data.