Abstract: A method of generating transcribed nucleic acids, comprising the steps of hybridizing an oligonucleotide probe to a nucleic acid template, wherein said oligonucleotide probe comprises a noncomplementary part in 5? direction of the complementary part that does not hybridize to the nucleic acid template and comprises a sequence of a transcription promoter, hydrolysing a 3? part of the nucleic acid template that is in 3? direction to a part of the nucleic acid template that hybridizes to the oligonucleotide probe and wherein said 3? part is not hybridized to the oligonucleotide probe, extending the nucleic acid template with nucleic acids complementary to the non-complementary part of the oligonucleotide probe, thereby generating a duplex of the sequence of the transcription promoter in sequence with the nucleic acid template, and transcribing the nucleic acid template with a transcriptase that binds the duplex of the sequence of the transcription promoter.