Abstract: Disclosed herein are methods, compositions, and kits related to the identification and/or quantification of target molecules.

Abstract: A method or platform for monoclonal antibody based biomarker discovery is disclosed. The method according to the invention provides for the integration of analyte collection, hybridoma screening and nanovolume integrated mass spectrometry (NVIMS) to achieve a robust screening system that is capable, for example, of cutting 4-6 years off of the classical biomarker discovery and development process. The invention provides a platform for the rapid, high-throughput production, isolation and characterization of, e.g., disease specific biomarkers together with highly specific monoclonal antibodies. The method of the invention has a variety of applications such as, but not limited to, drug testing, biohazard applications, ecological applications, physiological applications and/or pathology screening applications. The method of the invention is also capable of being performed or used as or with a high-throughput screening process or system of the invention.

Abstract: A method or platform for monoclonal antibody based biomarker discovery is disclosed. The method according to the invention provides for the integration of analyte collection, hybridoma screening and nanovolume integrated mass spectrometry (NVIMS) to achieve a robust screening system that is capable, for example, of cutting 4-6 years off of the classical biomarker discovery and development process. The invention provides a platform for the rapid, high-throughput production, isolation and characterization of, e.g., disease specific biomarkers together with highly specific monoclonal antibodies. The method of the invention has a variety of applications such as, but not limited to, drug testing, biohazard applications, ecological applications, physiological applications and/or pathology screening applications. The method of the invention is also capable of being performed or used as or with a high-throughput screening process or system of the invention.

Abstract: Methods and systems for comparing a first sequence and a second sequence, including associating errors with alignments of the first sequence and the second sequence, comparing the alignment errors to identify the alignment having the smallest error, and, based on the alignment having the smallest error, computing: a first percent identity relative to the first sequence, and a second percent identity relative to the second sequence.

Abstract: A biotinylated protein is disclosed which is obtained from the seeds of leguminous plants and which is expressed exclusively in the seeds and in no other tissue. The protein comprises at least one subunit of 50-85 kDa. Levels of the protein decrease rapidly as germination of the seed progresses. The protein does not exhibit the activity of either acetyl-CoA carboxylase or 3-methyl crotonyl-CoA carboxylase. In the pea, Pisum sativum, the protein is designated SBP65 and comprises 6-8 identical subunits, each having a molecular weight of about 65 kDa. The protein may be a useful marker for determining the germination stage of seeds.