Abstract: The present invention discloses a highly efficient method for detecting opioids, opiates, cannabinoids, or benzodiazepines present in a sample, comprising the steps of adding to said sample an enzyme with ?-glucuronidase activity originated from genus Brachyspira or any mutant derived thereof; incubating the sample with the enzyme; and detecting said opioids, opiates, cannabinoids, or benzodiazepines by means of a suitable technique.

Abstract: The present invention discloses a highly efficient method for detecting opiods, opiates, cannabinoids, or benzodiazepines present in a sample, comprising the steps of adding to said sample an enzyme with ?-glucuronidase activity originated from genus Brachyspira or any mutant derived thereof; incubating the sample with the enzyme; and detecting said opiods, opiates, cannabinoids, or benzodiazepines by means of a suitable technique.

Abstract: The present invention provides an insoluble enzymatic reagent for detecting products derived from glucuronide metabolites in a sample, containing an enzyme with glucuronidase activity immobilized on a resin comprising a polysaccharide modified with chemical groups, and optionally a cationic polymer. Additionally, it provides methods for preparing said enzymatic reagent and for detecting derivatives of glucuronide metabolites in a sample, using said reagent.

Abstract: The present invention provides an insoluble enzymatic reagent for detecting products derived from glucuronide metabolites in a sample, containing an enzyme with glucuronidase activity immobilized on a resin comprising a polysaccharide modified with chemical groups, and optionally a cationic polymer. Additionally, it provides methods for preparing said enzymatic reagent and for detecting derivatives of glucuronide metabolites in a sample, using said reagent.

Abstract: The present invention discloses a method and a highly efficient reagent to detect products derived from glucuronide metabolites presents in a sample, comprising the steps of adding to said sample an enzyme with ?-glucuronidase activity originated from genus Brachyspira or any variant or mutant derived thereof; incubating the sample with the enzyme during a determined period of time; and detecting the product derived from glucuronide metabolite by means of a suitable technique. The reagent of the present invention comprises an enzyme with ?-glucuronidase activity originated from genus Brachyspira or any variant or mutant derived thereof; and a suitable vehicle.