Abstract: Biological sample processing system assemblies include a swab chamber assembly configured to enhance transfer of a biological sample on a swab head to a resulting biological sample solution for subsequent processing. A swab chamber assembly includes a fluid channel and an elongated swab chamber having a proximal portion and a distal portion. The swab chamber is configured to accommodate insertion of a swab head of a swab into the swab chamber. The distal portion of the swab chamber slopes downwardly from a distal end of the proximal portion of the swab chamber so that lysis buffer solution transferred into the swab chamber accumulates within the distal portion of the swab chamber.

Abstract: Diagnostic testing systems and related methods employ actuation pressure-based control during fluid transfer within a test cartridge. A method of operating a cartridge to process a biological sample includes coupling an actuation port of the cartridge with a pump assembly of a portable analyzer. A transfer operation of the pump assembly induces airflow through the actuation port so as to displace an aliquot of the sample solution along a fluid channel. A control unit monitors a pressure signal indicative of a pressure of air displaced by the pump assembly. The transfer operation is stopped in response to a change in the pressure signal indicative of contact of the aliquot of the sample solution with a hydrophobic staging vent.

Abstract: Single use cartridges for testing biological samples are provided that include multiple processing channels. A single use cartridge includes an aliquot staging well, a first amplification well, a second amplification well, an actuation port, and a fluid channel control valve assembly. The fluid channel control valve assembly is reconfigurable between a first fluid channel configuration and a second fluid channel configuration.