Patents by Inventor Marc P. Raphael
Marc P. Raphael has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11702622Abstract: A system and method for studying cell injury mechanisms by applying biologically relevant mechanical impact to in vitro cell culture are disclosed. This approach is for maintaining consistent in vitro conditions during experiments, accommodating multiple cell populations, and monitoring each in real-time while achieving amplitude and time scale of input acceleration that mimic blunt injury cases. These multiplexed, environmental control capabilities enable characterizing the relationships between mechanical impact and cell injury in multivariate biological systems.Type: GrantFiled: August 21, 2020Date of Patent: July 18, 2023Assignee: The Government of the United States of America, as represented by the Secretary of the NavyInventors: Marc P. Raphael, Wonmo Kang
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Publication number: 20210341474Abstract: A method for the spatiotemporal mapping of receptor-ligand binding kinetics in localized surface plasmon resonance (LSPR) imaging using a chip for LSPR imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array. The nanostructures can be regenerated allowing the chip to be used multiple times.Type: ApplicationFiled: July 17, 2021Publication date: November 4, 2021Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
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Patent number: 11067573Abstract: A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.Type: GrantFiled: January 29, 2018Date of Patent: July 20, 2021Assignee: The Government of the United States of America, as represented by the Secretary of the NavyInventors: Marc P. Raphael, Joseph A Christodoulides, Jeff M Byers
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Publication number: 20210054325Abstract: A system and method for studying cell injury mechanisms by applying biologically relevant mechanical impact to in vitro cell culture are disclosed. This approach is for maintaining consistent in vitro conditions during experiments, accommodating multiple cell populations, and monitoring each in real-time while achieving amplitude and time scale of input acceleration that mimic blunt injury cases. These multiplexed, environmental control capabilities enable characterizing the relationships between mechanical impact and cell injury in multivariate biological systems.Type: ApplicationFiled: August 21, 2020Publication date: February 25, 2021Inventors: Marc P. Raphael, Wonmo Kang
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Patent number: 10761028Abstract: Methods and systems for determining extracellular concentration data of an analyte are disclosed. A method for determining extracellular concentration data of an analyte includes receiving sensor data from one or more arrays of functionalized plasmonic nanostructures on a localized surface plasmon resonance imaging chip in contact with a fluid containing at least one living cell for a plurality of times, determining intensity data for the one or more arrays, determining fractional occupancy based on the intensity data, and determining extracellular concentration data based on the fractional occupancy data. A system for determining extracellular concentration data of an analyte includes a LSPRi chip, a sensor component, an intensity component, a fractional occupancy component, a concentration component, and a processor to implement the components.Type: GrantFiled: June 20, 2016Date of Patent: September 1, 2020Assignee: The Government of the United States of America, as represented by the Secretary of the NavyInventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
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Publication number: 20200158639Abstract: Methods and systems for determining extracellular concentration data of an analyte are disclosed. A method for determining extracellular concentration data of an analyte includes receiving sensor data from one or more arrays of functionalized plasmonic nanostructures on a localized surface plasmon resonance imaging chip in contact with a fluid containing at least one living cell for a plurality of times, determining intensity data for the one or more arrays, determining fractional occupancy based on the intensity data, and determining extracellular concentration data based on the fractional occupancy data. A system for determining extracellular concentration data of an analyte includes a LSPRi chip, a sensor component, an intensity component, a fractional occupancy component, a concentration component, and a processor to implement the components.Type: ApplicationFiled: January 21, 2020Publication date: May 21, 2020Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
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Patent number: 10641705Abstract: A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.Type: GrantFiled: October 16, 2017Date of Patent: May 5, 2020Assignee: The Government of the United States of America, as represented by the Secretary of the NavyInventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
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Patent number: 10345287Abstract: A method for calibrating multiple nanostructures in parallel for quantitative biosensing using a chip for localized surface plasmon resonance (LSPR) biosensing and imaging. The chip is a glass coverslip compatible for use in a standard microscope with at least one array of functionalized plasmonic nanostructures patterned onto it using electron beam nanolithography. The chip is used to collect CCD-based LSPR imagery data of each individual nanostructure and LSPR spectral data of the array. The spectral data is used to determine the fractional occupancy of the array. The imagery data is modeled as a function of fractional occupancy to determine the fractional occupancy of each individual nanostructure.Type: GrantFiled: September 27, 2013Date of Patent: July 9, 2019Assignee: The United States of America, as represented by the Secretary of the NavyInventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
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Publication number: 20190162662Abstract: A method for measuring surface-induced cellular behavior that includes one or more lithographically patterned, functionalizable structures on a substrate, for example gold islands or grooved quartz, in contact with a fluid and in registry with at least one living cell for a plurality of times. The structures' shape, height, pitch and ordering are controlled by the lithographic process, such that the physical cues imparted to the cell by topography can be tuned independently of the chemical biofunctionality which is subsequently imparted via surface chemistry. Cellular behavior data, such as adhesion, migration, differentiation, division, secretion, apoptosis and necrosis, is measured using imaging sensors in relation to the surface topography and surface chemistry for a plurality of times.Type: ApplicationFiled: November 20, 2018Publication date: May 30, 2019Inventors: Marc P. Raphael, Joseph A. Christodoulides, Marc Christophersen, Jeff M. Byers
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Publication number: 20180164309Abstract: A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.Type: ApplicationFiled: January 29, 2018Publication date: June 14, 2018Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
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Patent number: 9915654Abstract: A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.Type: GrantFiled: September 27, 2013Date of Patent: March 13, 2018Assignee: The United States of America, as represented by the Secretary of the NavyInventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
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Publication number: 20180038791Abstract: A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.Type: ApplicationFiled: October 16, 2017Publication date: February 8, 2018Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
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Patent number: 9791368Abstract: A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.Type: GrantFiled: March 13, 2014Date of Patent: October 17, 2017Assignee: The United States of America as represented by the Secretary of the NavyInventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
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Patent number: 9778265Abstract: Genetic fusions of proteins, for example single-domain antibodies (sdAbs), with a positively-charged domain enhanced immobilization of active protein in a desired orientation.Type: GrantFiled: April 16, 2015Date of Patent: October 3, 2017Assignee: The United States of America, as represented by the Secretary of the NavyInventors: George P. Anderson, Jinny Lin Liu, Marc P. Raphael, Ellen R. Goldman
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Publication number: 20160370290Abstract: Methods and systems for determining extracellular concentration data of an analyte are disclosed. A method for determining extracellular concentration data of an analyte includes receiving sensor data from one or more arrays of functionalized plasmonic nanostructures on a localized surface plasmon resonance imaging chip in contact with a fluid containing at least one living cell for a plurality of times, determining intensity data for the one or more arrays, determining fractional occupancy based on the intensity data, and determining extracellular concentration data based on the fractional occupancy data. A system for determining extracellular concentration data of an analyte includes a LSPRi chip, a sensor component, an intensity component, a fractional occupancy component, a concentration component, and a processor to implement the components.Type: ApplicationFiled: June 20, 2016Publication date: December 22, 2016Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
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Publication number: 20160305948Abstract: Genetic fusions of proteins, for example single-domain antibodies (sdAbs), with a positively-charged domain enhanced immobilization of active protein in a desired orientation.Type: ApplicationFiled: April 16, 2015Publication date: October 20, 2016Applicant: The Govemment of The US, as represented by the Secretary of the NavyInventors: George P. Anderson, Jinny Lin Liu, Marc P. Raphael, Ellen R. Goldman
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Publication number: 20140273002Abstract: A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.Type: ApplicationFiled: March 13, 2014Publication date: September 18, 2014Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
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Publication number: 20140095100Abstract: A method for calibrating multiple nanostructures in parallel for quantitative biosensing using a chip for localized surface plasmon resonance (LSPR) biosensing and imaging. The chip is a glass coverslip compatible for use in a standard microscope with at least one array of functionalized plasmonic nanostructures patterned onto it using electron beam nanolithography. The chip is used to collect CCD-based LSPR imagery data of each individual nanostructure and LSPR spectral data of the array. The spectral data is used to determine the fractional occupancy of the array. The imagery data is modeled as a function of fractional occupancy to determine the fractional occupancy of each individual nanostructure.Type: ApplicationFiled: September 27, 2013Publication date: April 3, 2014Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers
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Publication number: 20140093977Abstract: A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.Type: ApplicationFiled: September 27, 2013Publication date: April 3, 2014Inventors: Marc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers