Patents by Inventor Marcel G. Wubbolts
Marcel G. Wubbolts has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9523083Abstract: Process for biochemical synthesis of 1,4-butanediannine in a microorganism having an increased level of an ornithine decarboxylase activity as compared to the native level of the ornithine decarboxylase activity, in which the increased ornithine decarboxylase activity is obtained by overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency. The process is performed by excreting 1,4-butanediamine produced in the microorganism into a fermentation broth, and recovering the 1,4-butanediannine from the fermentation broth. The increased translational and/or transcriptional efficiency is obtained by the use of a strong, regulated promoter consisting of an isopropvl-B-D-thiogalactoside (IPTG) inducible strong promoter.Type: GrantFiled: July 11, 2005Date of Patent: December 20, 2016Assignee: DSM IP ASSETS B.V.Inventors: Katrin Eppelmann, Petrus M. M. Nossin, Susanne M. Kremer, Marcel G. Wubbolts
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Patent number: 8497098Abstract: The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein in the microorganism also an increased activity of N-acetylglutamate formation is present as compared to the native level of activity of N-acetylglutamate formation in the microorganism and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. The invention also relates to vectors, plasmids and hosts carrying a corresponding increased ODC activity and an increased activity of N-acetylglutamate formation.Type: GrantFiled: July 11, 2005Date of Patent: July 30, 2013Assignee: DSM IP Assets B.V.Inventors: Katrin Eppelmann, Petrus M. M. Nossin, Leon J. R. M. Raeven, Susanne M. Kremer, Marcel G. Wubbolts
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Publication number: 20100068775Abstract: The present invention relates to the use of polynucleotides and polypeptides as biotechnological tools in the production of hydroxytyrosol from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of hydroxytyrosol in said microorganism. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the production of hydroxytyrosol.Type: ApplicationFiled: November 27, 2007Publication date: March 18, 2010Inventors: Jihane Achkar, Abel Ferrandez, Theodorus Sonke, Marcel G. Wubbolts
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Publication number: 20090275093Abstract: The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein the increased ODC activity is obtained by means of overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency, and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. In preferred embodiments also increased enzyme activity is obtained by of overexpression of either (i) an arginine decarboxylase encoding gene speA and an agmatinase encoding gene speB; or (ii) an arginine decarboxylase encoding gene speA and an agmatine iminohydrolase encoding gene aguA, and an N-carbamoylputrescine amidohydrolase encoding gene aguB, and optionally also an agmatinase encoding gene speB.Type: ApplicationFiled: July 11, 2005Publication date: November 5, 2009Inventors: Katrin Eppelmann, Petrus M. M. Nossin, Susanne M. Kremer, Marcel G. Wubbolts
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Publication number: 20090137759Abstract: The invention relates to biochemical synthesis of 6-amino caproic acid from 6-aminohex-2-enoic acid compound or from 6-amino-2-hydroxyhexanoic acid, by treatment with an enzyme having ?,?-enoate reductase activity towards molecules containing an ?,?-enoate group and a primary amino group. The invention also relates to processes for obtaining suitable genetically engineered cells for being used in such biotransformation process, and to precursor fermentation of 6-amino caproic acid from intermediates leading to 6-amino caproic acid. Finally, the invention relates to certain novel biochemically produced compounds, namely 6-aminohex-2-enoic acid, 6-aminohexanoic acid, as well as to caprolactam produced therefrom and to nylon-6 and other derivatives produced from such biochemically produced compounds or caprolactam.Type: ApplicationFiled: January 8, 2009Publication date: May 28, 2009Applicant: DSM IP ASSETS B.V.Inventors: Petronella C. Raemakers-Franken, Petrus M.M. Nossin, Paul M. Brandts, Marcel G. Wubbolts, Wijnand P.H. Peeters, Sandra Ernste, Stefaan M.A. Wildeman De, Martin Schuermann
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Patent number: 7491520Abstract: The invention relates to biochemical synthesis of 6-amino caproic acid from 6-aminohex-2-enoic acid compound or from 6-amino-2-hydroxyhexanoic acid, by treatment with an enzyme having ?,?-enoate reductase activity towards molecules containing an ?,?-enoate group and a primary amino group. The invention also relates to processes for obtaining suitable genetically engineered cells for being used in such biotransformation process, and to precursor fermentation of 6-amino caproic acid from intermediates leading to 6-amino caproic acid. Finally, the invention relates to certain novel biochemically produced compounds, namely 6-aminohex-2-enoic acid, 6-aminohexanoic acid, as well as to caprolactam produced therefrom and to nylon-6 and other derivatives produced from such biochemically produced compounds or caprolactam.Type: GrantFiled: January 17, 2005Date of Patent: February 17, 2009Assignee: DSM IP Assets B.V.Inventors: Petronella C. Raemakers-Franken, Petrus M. M. Nossin, Paul M. Brandts, Marcel G. Wubbolts, Wijnand P. H. Peeters, Sandra Ernste, Stefaan M. A. Wildeman De, Martin Schuermann
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Publication number: 20090011478Abstract: The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein in the microorganism also an increased activity of N-acetylglutamate formation is present as compared to the native level of activity of N-acetylglutamate formation in the microorganism and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. The invention also relates to vectors, plasmids and hosts carrying a corresponding increased ODC activity and an increased activity of N-acetylglutamate formation.Type: ApplicationFiled: July 11, 2005Publication date: January 8, 2009Applicant: DSM IP ASSETS B.V.Inventors: Katrin Eppelmann, Petrus M.M. Nossin, Leon J.R.M. Raeven, Susanne M. Kremer, Marcel G. Wubbolts