Patents by Inventor Marcel Reichen
Marcel Reichen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240042442Abstract: The invention relates to a microfluidic system comprising: a) a solid support comprising at least a first group of oligonucleotides, i. wherein each oligonucleotide in said group comprises a nucleic acid sequence of a first type, of a second type and/or a further type, ii. wherein said nucleic acid sequence of a first type is a barcode sequence, iii. and oligonucleotides comprising the same barcode sequence are grouped together in a group of oligonucleotides on said solid support, iv. wherein the first and further oligonucleotide groups are spatially separated on said solid support, b) wherein said one or more groups of oligonucleotide groups on said solid support are within separate reservoirs of the microfluidics system, c) wherein the one or more reservoirs are accessible to fluids, cells, chemicals and/or microdroplet by means of channels, and d) wherein each reservoir comprises comprising a group of oligonucleotides on said solid support is also trap for a microfluidic droplet.Type: ApplicationFiled: December 17, 2021Publication date: February 8, 2024Inventors: Sami Ellouze, Bingqing Shen, Arnaud Reitz, Annabelle Patricia Veronique Gérard, Karolis Simutis, Andrew Griffiths, Marcel Reichen
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Patent number: 11738344Abstract: The invention relates to a method for ordering, sorting and/or focusing particles in a first microfluidic channel system, the method comprising the steps of i) providing for a first microfluidic channel comprising at least a first and a second inlet and a first outlet, ii) injecting a first fluid into the channel through said first inlet, iii) injecting a second fluid into the channel through said second inlet, wherein the viscosity of the first fluid is higher than the viscosity of the second fluid, such that the two fluids flow in a laminar fashion unmixed side by side, and one of the two fluids comprises the particles to be ordered, sorted and/or focused. The invention also relates to a microfluidic channel system for sorting different particles into one droplet.Type: GrantFiled: September 19, 2018Date of Patent: August 29, 2023Assignee: HIFIBIO SASInventors: Raphael Clément Li-Ming Doineau, Arnaud Reitz, Marcel Reichen
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Publication number: 20230194396Abstract: The present invention relates to a method for analyzing and selecting a specific droplet among a plurality of droplets (4), comprising the following steps: —providing a plurality of droplets (4), —for a droplet (4) among the plurality of droplets, measuring at least two optical signals, each optical signal being representative of a light intensity spatial distribution in the droplet for an associated wavelength channel, —calculating a plurality of parameters from the optical signals, —determining a sorting class for a droplet according to calculated parameters, —sorting said droplet according to its sorting class, wherein the plurality of parameters comprises the coordinates of a maximum for each optical signal and a co-localization parameter and the at least two calculated parameters used for the determining step comprises the co-localization parameter.Type: ApplicationFiled: February 22, 2023Publication date: June 22, 2023Inventors: Marcel Reichen, Raphael Clément Li-ming Doineau, Sami Ellouze
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Publication number: 20230063190Abstract: The present invention concerns processes for barcoding nucleic acids from single cells and processes for genotyping ingle cells having a phenotype of interest.Type: ApplicationFiled: August 26, 2022Publication date: March 2, 2023Inventors: Marcel Reichen, Arnaud Reitz, Bingqing Shen, Sami Ellouze, Annabelle Gérard
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Patent number: 11592367Abstract: The present invention relates to a method for analyzing and selecting a specific droplet among a plurality of droplets (4), comprising the following steps: —providing a plurality of droplets (4), —for a droplet (4) among the plurality of droplets, measuring at least two optical signals, each optical signal being representative of a light intensity spatial distribution in the droplet for an associated wavelength channel, —calculating a plurality of parameters from the optical signals, —determining a sorting class for a droplet according to calculated parameters, —sorting said droplet according to its sorting class, wherein the plurality of parameters comprises the coordinates of a maximum for each optical signal and a co-localization parameter and the at least two calculated parameters used for the determining step comprises the co-localization parameter.Type: GrantFiled: January 18, 2018Date of Patent: February 28, 2023Assignee: HIFIBIO SASInventors: Marcel Reichen, Raphael Clément Li-Ming Doineau, Sami Ellouze
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Patent number: 11427853Abstract: The present invention concerns processes for barcoding nucleic acids from single cells and processes for genotyping single cells having a phenotype of interest.Type: GrantFiled: March 15, 2018Date of Patent: August 30, 2022Assignee: HIFIBIO SASInventors: Marcel Reichen, Arnaud Reitz, Bingqing Shen, Sami Ellouze, Annabelle Gerard
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Publication number: 20210252509Abstract: A multifunctional dual mode microfluidic device including a first operating configuration and a second operating configuration wherein in the first operating configuration, the device is configured to perform static cell seeding or produce cells. In the second operating configuration, the device is configured to perform perfusion via microfluidics of the device, and the device is configured to switch between the first operating configuration and the second operating configuration. The first operating configuration and the second operating configuration are selectively and independently accessible, wherein the first operating configuration or the second operating configuration does not alter any other configuration of the device. The device is fully operable in either one of the first operating configuration or the second operating configuration.Type: ApplicationFiled: April 30, 2021Publication date: August 19, 2021Inventors: Nicolas Szita, Marcel Reichen
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Publication number: 20210187508Abstract: The present invention generally relates to microfluidic droplets and, in particular, to multiple emulsion microfluidic droplets. Provided are methods and a device of ordering, sorting and/or focusing particles, the method comprising leading the particles through a microfluidic channel comprising a channel height (H) in the range of (1.8) D to (1.2) D and a channel width (W) in the range of (1.33) D to 1 D, wherein D is the particle diameter.Type: ApplicationFiled: October 16, 2018Publication date: June 24, 2021Inventors: Steven Scherr, Colin J.H. Brenan, Michael J. Brenan, Marcel Reichen
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Patent number: 11033897Abstract: The invention relates to a microfluidic device including a chamber having a fluid inlet, a fluid outlet and a sealable port. In some embodiments, the fluid inlet and the fluid outlet may be positioned to direct fluid flowing from the fluid inlet to the fluid outlet through the chamber. Various embodiments may include a sealable port which may be aligned with the chamber to allow material to be placed directly into, or removed from, the chamber from the exterior of the device when the sealable port is open, and to inhibit and/or prevent fluid escaping through the sealable port when the port is sealed.Type: GrantFiled: May 6, 2015Date of Patent: June 15, 2021Inventors: Nicolas Szita, Marcel Reichen
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Publication number: 20210154674Abstract: The invention relates to a process for manufacturing a microfluidic chip comprising a solid material obtained from a sol-gel solution, the process comprising successively: a) casting a sol-gel solution made with tetraethyl orthosilicate onto a mold presenting a relief pattern and having a different thickness over the whole of the mold; b) gelling the sol-gel solution; c) unmolding and drying the gel obtained in b), so as to obtain a solid glass; and d) bonding said solid glass to a support, so as to obtain the microfluidic chip.Type: ApplicationFiled: May 18, 2018Publication date: May 27, 2021Inventors: Arnaud Reitz, Marcel Reichen, Bingqing Shen, Sami Ellouze
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Publication number: 20200338552Abstract: The present invention is directed to a fluidic interface for delivering a fluid into a microfluidic device, the interface comprising a chamber comprising a first accepting/delivering fluid opening and a second accepting/delivering fluid opening, each opening being positioned opposite to the other, wherein the diameter of the first accepting/delivering fluid opening is smaller than the internal diameter of the chamber and a tubing system connecting the first accepting/delivering fluid opening to a pressure source capable of generating positive or negative pressure. The interface is characterized in that the second accepting/delivering fluid opening is designed to mechanically limit its insertion into a receptacle of a microfluidic device to ensure a predetermined gap (H) between the second accepting/delivering fluid opening and a lower side of the receptacle of a microfluidic device.Type: ApplicationFiled: October 24, 2018Publication date: October 29, 2020Inventors: Steven Scherr, Colin J. H. Brenan, Michael J. Brenan, Marcel Reichen, Raphael Clement Li-Ming Doineau
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Publication number: 20200215545Abstract: The invention relates to a method for ordering, sorting and/or focusing particles in a first microfluidic channel system, the method comprising the steps of i) providing for a first microfluidic channel comprising at least a first and a second inlet and a first outlet, ii) injecting a first fluid into the channel through said first inlet, iii) injecting a second fluid into the channel through said second inlet, wherein the viscosity of the first fluid is higher than the viscosity of the second fluid, such that the two fluids flow in a laminar fashion unmixed side by side, and one of the two fluids comprises the particles to be ordered, sorted and/or focused. The invention also relates to a microfluidic channel system for sorting different particles into one droplet.Type: ApplicationFiled: September 19, 2018Publication date: July 9, 2020Inventors: Raphael Clément Li-Ming Doineau, Arnaud Reitz, Marcel Reichen
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Publication number: 20200199649Abstract: The present invention concerns processes for barcoding nucleic acids from single cells and processes for genotyping single cells having a phenotype of interest.Type: ApplicationFiled: March 15, 2018Publication date: June 25, 2020Inventors: Marcel Reichen, Arnaud Reitz, Bingqing Shen, Sami Ellouze, Annabelle Gerard
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Publication number: 20190376879Abstract: The present invention relates to a method for analyzing and selecting a specific droplet among a plurality of droplets (4), comprising the following steps: —providing a plurality of droplets (4), —for a droplet (4) among the plurality of droplets, measuring at least two optical signals, each optical signal being representative of a light intensity spatial distribution in the droplet for an associated wavelength channel, —calculating a plurality of parameters from the optical signals, —determining a sorting class for a droplet according to calculated parameters, —sorting said droplet according to its sorting class, wherein the plurality of parameters comprises the coordinates of a maximum for each optical signal and a co-localization parameter and the at least two calculated parameters used for the determining step comprises the co-localization parameter.Type: ApplicationFiled: January 18, 2018Publication date: December 12, 2019Inventors: Marcel Reichen, Raphael Clément Li-Ming Doineau, Sami Ellouze
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Publication number: 20190345537Abstract: The present invention concerns a method for capturing and barcoding nucleic acid from single cells, a plurality of microfluidic droplets and a method for preparing said plurality of microfluidic droplets.Type: ApplicationFiled: December 8, 2016Publication date: November 14, 2019Inventors: Annabelle Patricia Véronique GERARD, Kévin Armand SELIN, Adeline Frédérique, Joëlle POITOU, Sami ELLOUZE, Yannick Michel, Louis POUSSE, Marcel REICHEN, Allan JENSEN, Colin Brenan
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Publication number: 20150231634Abstract: The invention relates to a microfluidic device including a chamber having a fluid inlet, a fluid outlet and a sealable port. In some embodiments, the fluid inlet and the fluid outlet may be positioned to direct fluid flowing from the fluid inlet to the fluid outlet through the chamber. Various embodiments may include a sealable port which may be aligned with the chamber to allow material to be placed directly into, or removed from, the chamber from the exterior of the device when the sealable port is open, and to inhibit and/or prevent fluid escaping through the sealable port when the port is sealed.Type: ApplicationFiled: May 6, 2015Publication date: August 20, 2015Inventors: Nicolas Szita, Marcel Reichen
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Publication number: 20110306081Abstract: The invention relates to a microfluidic device including a chamber having a fluid inlet, a fluid outlet and a sealable port. In some embodiments, the fluid inlet and the fluid outlet may be positioned to direct fluid flowing from the fluid inlet to the fluid outlet through the chamber. Various embodiments may include a sealable port which may be aligned with the chamber to allow material to be placed directly into, or removed from, the chamber from the exterior of the device when the sealable port is open, and to inhibit and/or prevent fluid escaping through the sealable port when the port is sealed.Type: ApplicationFiled: May 26, 2011Publication date: December 15, 2011Inventors: Nicolas Szita, Marcel Reichen