Abstract: A molecular probe comprising at least one first moiety and at least one second moiety which are each covalently bound to the molecular probe, wherein: the at least one first moiety comprises at least one binding peptide or peptidomimetic for binding the molecular probe to at least one extracellular vesicle (EV) membrane, wherein the binding is mediated by EV membrane curvature sensing; and the at least one second moiety comprises at least one support binding group for binding of the molecular probe to at least one support. The molecular probe can also comprise a spacer moiety, and/or a labeling moiety, and/or a modification moiety. The molecular probe can be bound to a solid or semi-solid support including a microarray support. The molecular probe can be used in methods in which it is contacted with sample which might include the extracellular vesicle and can involve detection and isolation steps. Kits can include the molecular probe.

Abstract: Provided herein are methods for capturing extracellular vesicles from a biological sample for quantification and/or characterization (e.g., size and/or shape discrimination) using an SP-IRIS system. Also provided herein are methods of detecting a biomarker on captured extracellular vesicles or inside the captured vesicles (e.g., intra-vesicular or intra-exosomal biomarkers).

Abstract: An assay for detecting gene fragments, including: amplifying gene fragments comprising single-nucleotide polymorphisms (SNPs) to form an initial amplification product; isolating single strand oligonucleotides of interest from the initial amplification product; forming a solution comprising the oligonucleotides of interest and reporter molecules, each reporter molecule having a first oligonucleotide domain configured to hybridize with a complementary oligonucleotide of interest, and a second oligonucleotide domain configured to hybridize with a complementary capture probe; hybridizing, in the solution, the oligonucleotides of interest with the reporter molecules that have complementary first domains; applying the solution to the surface of a microarray including an array of capture probes fixed to a microarray slide; capturing the oligonucleotides of interest on the microarray by hybridizing the second oligonucleotide domains of the reporter molecules with complementary capture probes of the microarray; and de

Abstract: Provided herein are methods for capturing extracellular vesicles from a biological sample for quantification and/or characterization (e.g., size and/or shape discrimination) using an SP-IRIS system. Also provided herein are methods of detecting a biomarker on captured extracellular vesicles or inside the captured vesicles (e.g., intra-vesicular or intra-exosomal biomarkers).

Abstract: The present invention relates to a method for the isolation of intact extracellular vesicles from biological tissues and fluids. Under a further aspect, the present invention relates intact extracellular vesicles, obtainable from the method herein described, and their use as a diagnostic and therapeutic tool.

Abstract: Provided herein are methods for capturing extracellular vesicles from a biological sample for quantification and/or characterization (e.g., size and/or shape discrimination) using an SP-IRIS system. Also provided herein are methods of detecting a biomarker on captured extracellular vesicles or inside the captured vesicles (e.g., intra-vesicular or intra-exosomal biomarkers).

Abstract: An assay method for a gene fragment of interest comprising: amplify at least one gene fragment of interest comprising or potentially comprising at least one SNP region of interest to form an initial amplification product; forming at least one single strand amplification product from the initial amplification product, wherein the single strand amplification product either comprises or potentially comprises the at least one SNP region of interest; hybridize in solution the single strand amplification product with at least one reporter molecule which comprises at least two, different domains of oligonucleotide, wherein a first domain of oligonucleotide is for hybridization with the single strand amplification product, and wherein the second domain of oligonucleotide is for hybridization to at least one microarray probe surface, wherein the microarray probe surface comprises at least one capture probe to allow for hybridization; contact the solution of the hybridized single strand amplification product with the a

Abstract: Novel copolymers provide for improved microarray performance. The copolymers are based on acrylamide, acrylate, and/or methacrylate monomer repeat units and include specific, functional monomers including a fluorinated monomer to control surface hydrophilicity such as increase water contact angle and reduce spot size when the copolymer is used in thin film form on a microarray substrate. The copolymers are prepared by free-radical polymerization and are random in nature.

Abstract: The invention relates to a method to reduce the electroosmotic flow in a capillary or in a channel and to a method to reduce the attachment of biological species to a surface comprising the step of coating said capillary, channel or surface with a copolymer comprising a surface interacting monomer, a monomer bearing a chemically active group and an ethylenically unsaturated silane monomer.

Abstract: The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support.

Abstract: Novel copolymers provide for improved microarray performance. The copolymers are based on acrylamide, acrylate, and/or methacrylate monomer repeat units and include specific, functional monomers including a fluorinated monomer to control surface hydrophilicity such as increase water contact angle and reduce spot size when the copolymer is used in thin film form on a microarray substrate. The copolymers are prepared by free-radical polymerization and are random in nature.

Abstract: Fabrication of arrays, including glycan arrays, that combines the higher sensitivity of a layered Si—SiO2 substrate with novel immobilization chemistry via a “click” reaction. The novel immobilization approach allows the oriented attachment of glycans on a “clickable” polymeric coating. The surface equilibrium dissociation constant (KD) of Concanavalin A with eight synthetic glycans was determined using fluorescence microarray. The sensitivity provided by the novel microarray substrate enables the evaluation of the influence of the glycan surface density on surface KD values. The interaction of carbohydrates with a variety of biological targets, including antibodies, proteins, viruses and cells are of utmost importance in many aspects of biology. Glycan microarrays are increasingly used to determine the binding specificity of glycan-binding proteins. The click polymers can be prepared in different forms such as soluble polymers, hydrogels, and multi-layers.

Abstract: The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support.

Abstract: The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support.

Abstract: The invention relates to a method to reduce the electroosmotic flow in a capillary or in a channel and to a method to reduce the attachment of biological species to a surface comprising the step of coating said capillary, channel or surface with a copolymer comprising a surface interacting monomer, a monomer bearing a chemically active group and an ethylenically unsaturated silane monomer.

Abstract: The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support.

Abstract: The instant invention describes uncharged water-soluble polymers to suppress electroosmotic flow in capillary electrophoresis. The polymers in the instant invention are copolymers of various derivatives of acrylamide and methacrylamide monomers with various glycidyl group containing monomers e.g.

Abstract: The present invention describes a method and apparatus utilizing a capillary coating of linear and crosslinked polymers and copolymers of acrylamidomonomers or methacrylamides bearing two hydroxyethyl residues. The method and apparatus employ this coating to maintain coating integrity and suppress electroosmotic flow under basic conditions in capillary electrophoresis.

Abstract: Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography are disclosed. The mixed-bed matrices are formed by the copolymerization of a substituted agarose mixture with acrylamide monomers. The disclosed matrices have improved polymerization properties enabling the formation of gels which have higher porosity and elasticity than conventional gels and which are therefore particularly suited to the separation of intermediate size DNA fragments and high molecular mass proteins.

Abstract: Matrices for electrokinetic and chromatographic separations, based on a unique class of mono- and disubstituted (on the amido nitrogen) acrylamides (e.g., acrylamido-N-ethoxy ethanol, acrylamido-N,N-diethoxy ethanol) off erthe following advantages: a) strong resistance to the alkaline hydrolysis (most zone separations occurring at basic pH values); b) a high hydrophilicity; and c) greater porosity, due to the larger M.sub.r value of the monomers. Such matrices can be prepared as `chemically crosslinked` gels or as `physical` gels, the latter consisting of strings of monofunctional monomer in the absence of crosslinker, at concentrations above the entanglement threshold. It is moreover possible to further increment the gel porosity by polymerizing it in the presence of laterally aggregating agents (e.g., polyethylene glycol of M.sub.r 6-20,000 Da), thus obtaining matrices which allow better resolution of large M.sub.