Patents by Inventor Marcellinus Gualbertus Hubertus Maria Beld

Marcellinus Gualbertus Hubertus Maria Beld has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Isolation and amplification of nucleic acid materials
    Patent number: 7238530
    Abstract: The invention relates to methods for easily separating single stranded nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at lease one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such a chelating agents, pH and the like, it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For very efficent separations, the process may be repeated.
    Type: Grant
    Filed: January 12, 2001
    Date of Patent: July 3, 2007
    Assignee: bioMerieux SA
    Inventors: Jaap Goudsmit, Cornelis Johannes Andreas Sol, Marcellinus Gualbertus Hubertus Maria Beld, Willem René Boom
  • Isolation and amplification of nucleic acid materials
    Publication number: 20010021518
    Abstract: The invention relates to methods for easily separating single stranded nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at lease one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such a chelating agents, pH and the like, it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For very efficent separations, the process may be repeated.
    Type: Application
    Filed: January 12, 2001
    Publication date: September 13, 2001
    Inventors: Jaap Goudsmit, Cornelis Johannes Andreas Sol, Marcellinus Gualbertus Hubertus Maria Beld, Willem Rene Boom