Patents by Inventor Marcello Marelli
Marcello Marelli has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210356377Abstract: The present invention is directed to methods of screening populations of transgenic cells for cells that produce a protein of interest. The methods comprise culturing transgenic cells in culture conditions that include at least one non-natural amino acid (nnAA) in the cell culture medium. The transgenic cells comprise at least one polynucleotide that codes for a fusion protein with a first domain coding for a protein of interest and a second domain coding for a domain that facilitates detection of the transgenic cells that express the protein of interest when the transgenic cell expresses the second domain.Type: ApplicationFiled: September 5, 2019Publication date: November 18, 2021Inventors: Zachary BRITTON, Marcello MARELLI, Michael A. BOWEN, Timothy LONDON, Li ZHUANG, Lina CHAKRABARTI
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Patent number: 10253345Abstract: There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.Type: GrantFiled: June 1, 2017Date of Patent: April 9, 2019Assignee: MEDIMMUNE LIMITEDInventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli, William Brady, Jeffrey C. Johnson
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Patent number: 10214761Abstract: There are provided amino acid derivatives of formula V and VI as defined herein which are pyrrolysine analogs for use in bioconjugation processes.Type: GrantFiled: June 1, 2017Date of Patent: February 26, 2019Assignee: MEDIMMUNE LIMITEDInventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli
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Patent number: 10131920Abstract: There is provided inter alia a DNA construct which comprises a tRNApyl coding sequence and a RNA polymerase III promoter sequence which is capable of acting to express functional tRNApyl sufficiently to support nonsense suppression in a eukaryotic expression system.Type: GrantFiled: March 14, 2014Date of Patent: November 20, 2018Assignee: MEDIMMUNE LIMITEDInventors: Giorgio Dieci, Marcello Marelli, Kenneth H. Grabstein
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Patent number: 9981908Abstract: There are provided pyrrolysine analogs of the formulae (X), (I), (II), (V), (VI), (VII) and (VIII), in which the a, b, d, X, Y, Z, FG, R, R1, R2 and R3 are as defined in the claims, which are useful in bioconjugation processes and mutant proteins containing them.Type: GrantFiled: August 4, 2014Date of Patent: May 29, 2018Assignee: MedImmune LimitedInventors: Marcello Marelli, Michael van Brunt, Kenneth H. Grabstein
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Publication number: 20170306380Abstract: There are provided amino acid derivatives of formula V and VI as defined herein which are pyrrolysine analogs for use in bioconjugation processes.Type: ApplicationFiled: June 1, 2017Publication date: October 26, 2017Inventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli
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Publication number: 20170306381Abstract: There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.Type: ApplicationFiled: June 1, 2017Publication date: October 26, 2017Inventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli, William Brady, Jeffrey C. Johnson
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Patent number: 9732367Abstract: There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.Type: GrantFiled: September 24, 2013Date of Patent: August 15, 2017Assignee: MEDIMMUNE LIMITEDInventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli, William Brady, Jeffrey C. Johnson
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Patent number: 9670521Abstract: There are provided amino acid derivatives of formula V and VI as defined herein which are pyrrolysine analogs for use in bioconjugation processes.Type: GrantFiled: September 24, 2013Date of Patent: June 6, 2017Assignee: MEDIMMUNE LIMITEDInventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli
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Publication number: 20160176811Abstract: There are provided pyrrolysine analogues of the formulae (X), (I), (II), (V), (VI), (VII) and (VIII), in which the a, b, d, X, Y, Z, FG, R, R1, R2 and R3 are as defined in the claims, which are useful in bioconjugation processes and mutant proteins containing them.Type: ApplicationFiled: August 4, 2014Publication date: June 23, 2016Inventors: Marcello Marelli, Michael van Brunt, Kenneth H. Grabstein
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Publication number: 20160024522Abstract: There is provided inter alia a DNA construct which comprises a tRNApyl coding sequence and a RNA polymerase III promoter sequence which is capable of acting to express functional tRNApyl sufficiently to support nonsense suppression in a eukaryotic expression system.Type: ApplicationFiled: March 14, 2014Publication date: January 28, 2016Inventors: Giorgio DIECI, Marcello MARELLI, Kenneth H. GRABSTEIN
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Publication number: 20150259721Abstract: There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.Type: ApplicationFiled: September 24, 2013Publication date: September 17, 2015Inventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli, William Brady, Jeffrey Johnson
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Publication number: 20150251994Abstract: There are provided amino acid derivatives of formula V and VI as defined herein which are pyrrolysine analogs for use in bioconjugation processes.Type: ApplicationFiled: September 24, 2013Publication date: September 10, 2015Inventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli
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Publication number: 20140106981Abstract: The present invention relates generally to methods for identifying and using organ-specific proteins and transcripts. The present invention further provides compositions comprising organ-specific proteins and transcripts encoding the same, detection reagents for detecting such proteins and transcripts, and diagnostic panels, kits and arrays for measuring organ-specific proteins/transcripts in blood, biological tissue or other biological fluid.Type: ApplicationFiled: November 5, 2013Publication date: April 17, 2014Applicants: Integrated Diagnostics, Inc., Institute for Systems BiologyInventors: Leroy Hood, Patricia M. Beckmann, Richard Johnson, Marcello Marelli, Xiaojun Li
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Patent number: 8586006Abstract: The present invention relates generally to methods for identifying and using organ-specific proteins and transcripts. The present invention further provides compositions comprising organ-specific proteins and transcripts encoding the same, detection reagents for detecting such proteins and transcripts, and diagnostic panels, kits and arrays for measuring organ-specific proteins/transcripts in blood, biological tissue or other biological fluid.Type: GrantFiled: August 9, 2007Date of Patent: November 19, 2013Assignees: Institute for Systems Biology, Integrated Diagnostics, Inc.Inventors: Leroy Hood, Patricia M. Beckmann, Richard Johnson, Marcello Marelli, Xiaojun Li
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Publication number: 20120087862Abstract: The present invention relates generally to methods for identifying and using organ-specific proteins and transcripts. The present invention further provides compositions comprising organ-specific proteins and transcripts encoding the same, detection reagents for detecting such proteins and transcripts, and diagnostic panels, kits and arrays for measuring organ-specific proteins/transcripts in blood, biological tissue or other biological fluid.Type: ApplicationFiled: August 9, 2007Publication date: April 12, 2012Applicants: HOMESTEAD CLINICAL CORPORATION, INSTITUTE FOR SYSTEMS BIOLOGYInventors: Leroy Hood, Patricia M. Beckmann, Richard Johnson, Marcello Marelli, Xiaojun Li