Abstract: The subject matter of this invention is a new thermolabile PPR nuclease or its enzymatically active fragment exhibiting high catalyst activity in difficult reaction conditions (particularly high concentrations of salts and other additives commonly used in the processes of the purification of proteins and viruses, low temperatures and wide range of pH), where the nuclease sequence is SEQ. 2 or a sequence that shares at least 40% of its identity. The subject matter of the invention is also the gene encoding PPR nuclease or its enzymatically active fragment; a particle of nucleic acid encoding the PPR nuclease or its enzymatically active fragment; expression plasmid including the sequence of the PPR encoding gene; recombinant strains of Escherichia coli JM109(DE3) pD454-PPR-AmpR and E. coli ArcticExpress (DE3) pD454-PPR-AmpR; the method of PPR nuclease protein production, application of PPR nuclease in processes of purification of recombinant proteins of significantly lower DNA content.

Abstract: The subject of the invention is the fusion single-stranded DNA polymerase Bst linked with NeqSSB protein at the N-end of the polymerase using the linker consisting of six amino acids with the amino acid sequence Gly-Ser-Gly-Gly-Val-Asp, wherein the given polymerase is present in three different variants, and the preparation method thereof. Moreover, the subject of the invention is the nucleic acid molecule encoding the fusion DNA polymerase NeqSSB-Bst Full Length, Large Fragment, Short Fragment and their utilisation.

Abstract: Embodiments provide application and/or resource access control features of an online computing environment, but are not so limited. In an embodiment, a computer-implemented method provides access control features for an online application environment based in part on the use of a number of directory service instances isolated from direct customer access and deployed in a defined datacenter architecture. In one embodiment, a computing environment uses web-based access control features and a number of directory service instances having organizational units and corresponding mappings to maintain a support infrastructure as part of providing features of online application services to customers. Other embodiments are included and available.

Abstract: Embodiments provide application and/or resource access control features of an online computing environment, but are not so limited. In an embodiment, a computer-implemented method provides access control features for an online application environment based in part on the use of a number of directory service instances isolated from direct customer access and deployed in a defined datacenter architecture. In one embodiment, a computing environment uses web-based access control features and a number of directory service instances having organizational units and corresponding mappings to maintain a support infrastructure as part of providing features of online application services to customers. Other embodiments are included and available.