Abstract: The present disclosure is directed to a recombinant nucleic acid construct encoding a defective interfering coronaviridae virus particle. The recombinant construct comprises: a nucleotide sequence encoding coronaviridae replication signals, wherein said nucleotide sequence of said recombinant nucleic acid construct does not encode one or more functional coronaviridae proteins. Defective interfering coronaviridae virus-like particles produced from the recombinant nucleic acid construct are also disclosed along with methods of treating a subject having a corona virus infection using the virus-like particle.

Abstract: The present invention relates to modified cancer cells that are defective in the production of diffusible factors (e.g., growth factors), and to the use of such cells for impairing the growth of a population of human cells, in particular wherein the cells are human cancer cells and the population of cells is a neoplasm, tumor or cancer. The invention preferably comprises the step of modifying tumor cells by deleting or modifying endogenous genes that code for (or affect the production of), diffusible growth factors that promote cell survival and proliferation.

Abstract: The present invention relates to modified cancer cells that are defective in the production of diffusible factors (e.g., growth factors), and to the use of such cells for impairing the growth of a population of human cells, in particular wherein the cells are human cancer cells and the population of cells is a neoplasm, tumour or cancer. The invention preferably comprises the step of modifying tumour cells by deleting or modifying endogenous genes that code for (or affect the production of), diffusible growth factors that promote cell survival and proliferation.