Abstract: The present invention provides engineered lysine decarboxylases that can be used to synthesize 1,5-diaminopentane under industrially relevant conditions. The present invention also provides polynucleotides encoding engineered lysine decarboxylases, host cells capable of expressing the engineered lysine decarboxylases, and methods for preparing 1,5-diaminopentane using the engineered lysine decarboxylases. The engineered lysine decarboxylase of the present invention was developed from a wild-type lysine decarboxylase through a creative process of directed evolution, and the engineered lysine decarboxylase of the present invention has a better activity and/or stability and tolerates a high substrate concentration compared to other lysine decarboxylases for the preparation of 1,5-diaminopentane, and thus has a good prospect for industrial application.

Abstract: A method for making intermediate compounds suitable for making muscenone, the compositions made by said methods, the various uses of said compositions, and enzyme variants useful in said methods.

Abstract: The present disclosure provides an engineered ketoreductase polypeptide capable of preparing chiral alcohols, especially for catalyzing the reduction of ethyl 4-chloroacetoacetate to produce ethyl (R)-4-chloro-3-hydroxybutyrate. The polypeptides has high catalytic activity, high stereoselectivity, better thermal stability and solvent tolerance; the reaction process using engineered ketoreductase polypeptides may have a substrate loading of up to 426 g/L and a conversion of up to 99%.

Abstract: Provided is an engineered polypeptide capable of catalyzing the asymmetric hydrolysis of 3-isobutylglutarimide to generate (R)-(?)-3-(carbamoylmethyl)-5-methylhexanoic acid. The engineered polypeptide has high stereoselectivity, high catalytic activity, good process stability and thermal stability, and tolerance to high product concentrations, and has good prospects for industrial applications.

Abstract: The present invention provides a computational method for designing artificial variants which have activity towards non-natural substrates. The present invention provides a special method to process stability evaluation results and creatively combines a process of calculating free energy barrier, which can improve the accuracy of the virtual screening of enzyme variants. The computational method disclosed by this invention greatly reduce the number of variants to be constructed and tested in the wet lab. In some cases, this method achieved the effect of enzyme engineering that cannot be achieved by traditional directed enzyme evolution methods.

Abstract: A method for making intermediate compounds suitable for making muscenone, the compositions made by said methods, the various uses of said compositions, and enzyme variants useful in said methods.

Abstract: The present invention provides ketoreductase variants having activity on both (R)-(?)-1,3-butanediol and (S)-(+)-1,3-butanediol, and the computational methodology for designing the above mentioned ketoreductase variants. The ketoreductase variants disclosed in the present application can catalyze the reaction of synthesizing 4-hydroxy-2-butanone from racemic 1,3-butanediol, which has industrial application prospects.

Abstract: Provided are amino acid sequences of ketoreductase polypeptides that are useful for asymmetrically synthesizing chiral alcohol compounds, its preparation process as well as reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered ketoreductase polypeptides, engineered host cells capable of expressing engineered ketoreductase polypeptides, and methods of producing chiral alcohol compounds using engineered ketoreductase polypeptides. Compared to other enzymes, the engineered ketoreductase polypeptides provided by the invention have better catalytic activity and thermal stability, allowing purification of the enzyme solution by heat treatment, which is advantageous for the production of enzymes and the industrial application of enzymatic reactions.

Abstract: Provided are amino acid sequences of engineered transaminase polynucleotide that are useful for asymmetrically synthesizing chiral amine compounds, and its preparation process and reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered transaminase polypeptides, engineered host cells capable of expressing engineered transaminase polypeptides, and methods of producing chiral amine compounds using engineered transaminase polypeptides. Compared to other enzymes, the engineered transaminase polypeptides provided by this invention have better catalytic activity and stability, and are not inhibited by chiral amine products in the synthesis process. The use of the engineered polypeptides of the present invention for the preparation of chiral amine compound results in higher unit activity, and has good industrial application prospects.

Abstract: A production equipment of phenethylamine includes a first reaction device; a second reaction device, wherein the second reaction device is connected to the first reaction device; a circulation device, wherein the circulation device is respectively connected to the first reaction device and the second reaction device; an acetone storage device; a centrifugal extraction device; a phenethylamine processing device; and a phenethylamine storage device. The first reaction device and the second reaction device have the same structure. A production method of phenethylamine is further provided, which uses transaminase as a catalyst, so that acetophenone and isopropylamine can flow through the transaminase to complete the reaction, the reaction is completed in one step, the production cycle is shortened, and the production cost is reduced.

Abstract: Provided are amino acid sequences of ketoreductase polypeptides that are useful for asymmetrically synthesizing chiral alcohol compounds, its preparation process as well as reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered ketoreductase polypeptides, engineered host cells capable of expressing engineered ketoreductase polypeptides, and methods of producing chiral alcohol compounds using engineered ketoreductase polypeptides. Compared to other enzymes, the engineered ketoreductase polypeptides provided by the invention have better catalytic activity and thermal stability, allowing purification of the enzyme solution by heat treatment, which is advantageous for the production of enzymes and the industrial application of enzymatic reactions.

Abstract: Provided are amino acid sequences of engineered transaminase polynucleotide that are useful for asymmetrically synthesizing chiral amine compounds, and its preparation process and reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered transaminase polypeptides, engineered host cells capable of expressing engineered transaminase polypeptides, and methods of producing chiral amine compounds using engineered transaminase polypeptides. Compared to other enzymes, the engineered transaminase polypeptides provided by this invention have better catalytic activity and stability, and are not inhibited by chiral amine products in the synthesis process. The use of the engineered polypeptides of the present invention for the preparation of chiral amine compound results in higher unit activity, and has good industrial application prospects.

Abstract: The subject of the invention is new membranes in which tailor-made membrane transport proteins (such as e.g. TCDB classified proteins) act as pore-forming proteins (e.g. FhuA) or peptides which act as pores in the membrane. The membranes can preferably be produced both by linking synthesised protein-polymer conjugates and by direct linking of the pore-forming proteins and peptides. Such membranes are distinguished by many outstanding features which existing membranes have not been able to offer to date.

Abstract: Novel glucose oxidase (GOx) variants are disclosed that have the substitutions of T30V and I94V set forth in SEQ ID NO:1, and additionally at least one further amino acid substitution in the enzyme sequence in any of the positions S53; A137; A173; A332; F414 and V560. The GOx variants herein exhibit specificity for glucose and significantly reduced oxygen consumption rates and/or increased enzyme activity for electron mediators other than oxygen. Also provided are assay devices incorporating at least one of the GOx variants herein for improved blood glucose measurements.

Abstract: Novel glucose oxidase variants are disclosed that have besides the two substitutions T30V and I94V according to SEQ ID NO:1, additionally at least one further amino acid substitution in the polypeptide sequence in any of the positions S53; A137; A173; A332; F414 and V560. The glucose oxidase variants herein exhibit specificity for glucose and significantly reduced oxygen consumption rates and/or increased enzyme activity for electron mediators other than oxygen. Also provided are assay devices incorporating at least one of the GOx variants herein for improved blood glucose measurements.

Abstract: The invention relates to a lipase variant which can be prepared by carrying out, on the amino acid sequence of a starting lipase selected from the lipase homologous family I.1 or I.2, at least one amino acid substitution in those positions which correspond to the positions 17, 29, 30, 52, 86, 117, 122, 160, 163, 167, 265, 266, 286, 289 in the prototype lipase sequence SEQ ID NO: 1.

Abstract: The invention relates to a lipase variant which can be prepared by carrying out, on the amino acid sequence of a starting lipase selected from the lipase homologous family I.1 or I.2, at least one amino acid substitution in those positions which correspond to the positions 17, 29, 30, 52, 86, 117, 122, 160, 163, 167, 265, 266, 286, 289 in the prototype lipase sequence SEQ ID NO: 1.