Patents by Inventor Marco Malten

Marco Malten has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200308509
    Abstract: The present invention relates to lipase variants and compositions comprising (i) at least one surfactant and (ii) at least one lipase variant of the invention. Furthermore, the present invention relates to methods of using the compositions.
    Type: Application
    Filed: June 26, 2017
    Publication date: October 1, 2020
    Applicant: NOVOZYMES A/S
    Inventors: Kim Borch, Carsten Hørslev Hansen, Jesper Vind, Marco Malten, Svend Gunnar Kaasgaard
  • Patent number: 10760037
    Abstract: The present invention relates to a composition comprising: (a) a source of sulfite; and (b) a lipase variant of a parent lipase, wherein said variant has improved stability towards sulfite as compared to the parent lipase.
    Type: Grant
    Filed: July 1, 2016
    Date of Patent: September 1, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Marco Malten, Thomas Agersten Poulsen, Lise Munch Mikkelsen, Dorte Marie Koefoed Klitgaard, Vibeke Skovgaard Nielsen, Katarina Larson
  • Publication number: 20200140965
    Abstract: The present invention relates to glucoamylase variants having an increase in specific activity and/or an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, at least 90%, at least 95% identity, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: October 18, 2019
    Publication date: May 7, 2020
    Applicant: NOVOZYMES A/S
    Inventors: Shikha Gupta, Allan Svendsen, Vivek Srivastava, Marco Malten, Thomas Agersten Poulsen, Preethi Ramaiya, Beth Nelson, Vasudeva Prahlada Rao, Padmavathi Balumuri
  • Patent number: 10494685
    Abstract: The present invention relates to glucoamylase variants having an increase in specific activity and/or an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, at least 90%, at least 95% identity, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: October 12, 2016
    Date of Patent: December 3, 2019
    Assignee: NOVOZYMES A/S
    Inventors: Shikha Gupta, Allan Svendsen, Vivek Srivastava, Marco Malten, Thomas Agersten Poulsen, Preethi Ramaiya, Beth Nelson, Vasudeva P. Rao, Padmavathi Balumuri
  • Publication number: 20190284541
    Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.
    Type: Application
    Filed: May 28, 2019
    Publication date: September 19, 2019
    Applicant: NOVOZYMES A/S
    Inventors: Marco Malten, Kim Borch, Lise Munch Mikkelsen, Thomas Agersten Poulsen, Luise Erlandsen, Hanne Philbert Nielsen, Carsten Horslev Hansen, Allan Svendsen, Christian Lundager Gylstorff, Jesper Vind, Christian Jackel
  • Patent number: 10351833
    Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.
    Type: Grant
    Filed: May 13, 2014
    Date of Patent: July 16, 2019
    Assignee: Novozymes A/S
    Inventors: Marco Malten, Kim Borch, Lise Munch Mikkelsen, Thomas Agersten Poulsen, Luise Erlandsen, Hanne Philbert Nielsen, Carsten Horslev Hansen, Allan Svendsen, Christian Lundager Gylstorff, Jesper Vind, Christian Jackel
  • Publication number: 20190071619
    Abstract: The present invention relates to a composition comprising: (a) a source of sulfite; and (b) a lipase variant of a parent lipase, wherein said variant has improved stability towards sulfite as compared to the parent lipase.
    Type: Application
    Filed: July 1, 2016
    Publication date: March 7, 2019
    Applicant: NOVOZYMES A/S
    Inventors: Marco Malten, Thomas Agersten Poulsen, Lise Munch Mikkelsen, Dorte Marie Koefoed Klitgaard, Vibeke Skovgaard Nielsen, Katarina Larson
  • Publication number: 20190048413
    Abstract: The present invention enables the determination by NGS of the full nucleotide sequence of one or more polynucleotides of interest that are more than 800 bp in length after only one round of PCR amplification and subsequent fragmentation by adding at least one known oligonucleotide index to at least one end of each polynucleotide to produce elongated polynucleotides, wherein each known oligonucleotide index added to each polynucleotide is unique; fragmenting the elongated polynucleotides; sequencing the resulting fragments with paired forward and reverse reads using bridge amplification and next-generation sequencing; sorting the paired sequences based on the known oligonucleotide; and performing manual or in silico assembly of the sorted paired sequences, thereby providing the full coding sequence of each of the multitude of polynucleotides.
    Type: Application
    Filed: February 23, 2017
    Publication date: February 14, 2019
    Applicant: NOVOZYMES A/S
    Inventors: Marco Malten, Thomas Agersten Poulsen, Preethi Ramaiya, Michael Bargisen, Beth Nelson
  • Publication number: 20180298456
    Abstract: The present invention relates to glucoamylase variants having an increase in specific activity and/or an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, at least 90%, at least 95% identity, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: October 12, 2016
    Publication date: October 18, 2018
    Applicant: NOVOZYMES A/S
    Inventors: Shikha Gupta, Allan Svendsen, Vivek Srivastava, Marco Malten, Thomas Agersten Poulsen, Preethi Ramaiya, Beth Nelson, Vasudeva P. Rao, Padmavathi Balumuri
  • Patent number: 10093911
    Abstract: The present invention relates to subtilisin variants and methods for obtaining subtilisin variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: October 9, 2018
    Assignee: Novozymes A/S
    Inventors: Werner Besenmatter, Marco Malten, Benie Astrid
  • Publication number: 20170321197
    Abstract: The present invention relates to a variant of a parent lipase, which parent lipase has the amino acid sequence of SEQ ID NO: 2, wherein the variant has lipase activity, comprises a substitution at a position corresponding to position 51 and comprises a substitution or remains unaltered at a position corresponding to position 96 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: December 8, 2015
    Publication date: November 9, 2017
    Applicant: Novozymes A/S
    Inventors: Christian Lundager Gylstorff, Christian Jackel, Jesper Vind, Allan Svendsen, Marco Malten, Thomas Agersten Poulsen
  • Patent number: 9809787
    Abstract: The present invention relates a method of obtaining a detergent composition comprising introducing (a) a lipase variant of a parent lipase which variant has at least 60% sequence identity with SEQ ID NO: 2, a substitution at a position corresponding to D254 of the mature polypeptide of SEQ ID NO: 2 and has lipase activity and (b) an anionic surfactant, wherein said composition has increased stability in comparison with a corresponding composition comprising the parent lipase.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: November 7, 2017
    Assignee: NOVOZYMES A/S
    Inventors: Jesper Vind, Lise Munch Mikkelsen, Marco Malten, Allan Svendsen, Kim Borch
  • Publication number: 20160076012
    Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.
    Type: Application
    Filed: May 13, 2014
    Publication date: March 17, 2016
    Applicant: Novozymes A/S
    Inventors: Marco Malten, Kim Borch, Lise Munch Mikkelsen, Thomas Agersten Poulsen, Luise Erlandsen, Hanne Philbert Nielsen, Carsten Horslev Hansen, Allan Svendsen, Christian Lundager Glystorff, Jesper Vind, Christian Jackel
  • Publication number: 20150031114
    Abstract: The present invention relates to detergent compositions comprising subtilisin variants and methods for obtaining such detergent compositions. The present invention also relates to the use of such detergent compositions, especially in laundry or in hard surface cleaning applications.
    Type: Application
    Filed: February 14, 2013
    Publication date: January 29, 2015
    Inventors: Werner Besenmatter, Astrid Benie, Marco Malten
  • Publication number: 20150004671
    Abstract: The present invention relates to subtilisin variants and methods for obtaining subtilisin variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: February 14, 2013
    Publication date: January 1, 2015
    Applicant: Novozymes A/S
    Inventors: Werner Besenmatter, Marco Malten, Benie Astrid
  • Publication number: 20140342433
    Abstract: The present invention relates to subtilase variants and methods for obtaining subtilase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: November 23, 2012
    Publication date: November 20, 2014
    Inventors: Allan Svendsen, Marco Malten, Christian Lundager Gylstorff
  • Patent number: 8883436
    Abstract: Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed.
    Type: Grant
    Filed: July 13, 2010
    Date of Patent: November 11, 2014
    Assignee: Novozymes A/S
    Inventors: Marco Malten, Keith McFarland
  • Publication number: 20140323382
    Abstract: The present invention relates a method of obtaining a detergent composition comprising introducing (a) a lipase variant of a parent lipase which variant has at least 60% sequence identity with SEQ ID NO: 2, a substitution at a position corresponding to D254 of the mature polypeptide of SEQ ID NO: 2 and has lipase activity and (b) an anionic surfactant, wherein said composition has increased stability in comparison with a corresponding composition comprising the parent lipase.
    Type: Application
    Filed: December 20, 2012
    Publication date: October 30, 2014
    Inventors: Jesper Vind, Lise Munch Mikkelsen, Marco Malten, Allan Svendsen, Kim Borch
  • Publication number: 20130224757
    Abstract: The present invention relates to a method of screening for an improved enzyme variant, said method comprising the steps of: (i) providing a recombinant host cell capable of sporulating comprising a polynucleotide encoding a sporulation factor and a polynucleotide encoding an enzyme variant which are operably linked to an inducible promoter; (ii) culturing the host cell under conditions suitable to induce the formation of spores; (iii) culturing the spores obtained in step (ii) in a medium containing a substrate for the enzyme variant; and (iv) determining the activity of the enzyme variant.
    Type: Application
    Filed: August 18, 2011
    Publication date: August 29, 2013
    Applicant: NOVOZYMES A/S
    Inventors: Mads Bjoernvad, Poul Erik Pedersen, Marco Malten
  • Publication number: 20120190054
    Abstract: Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed.
    Type: Application
    Filed: July 13, 2010
    Publication date: July 26, 2012
    Applicant: NOVOZYMES A/S
    Inventors: Marco Malten, Keith McFarland