Abstract: Detection method for sample presence and device associated The invention relates to a detection method of liquid presence inside a well of a transparent or translucent support intended for analysis performed in a device for in vitro detection and/or quantification, the presence of liquid being assessed on the basis of an index of visible pattern likelihood representative of the probability of a pattern to be legible as expected in the field of view of an imager. Said invention allows preventing assay execution without sample and can be applied without wasting sample or sample well support in case of the sample well is not filled by the user. Moreover, this method allows a precise detection of the samples that are transparent or translucent, with low reflectance.