Patents by Inventor Marcos Castanheira Alegria

Marcos Castanheira Alegria has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Animal product-free culture medium for bacteria of the genus and a process for producing supernatant comprising one or more collagenolytic and gelatinolytic proteases
    Patent number: 10597632
    Abstract: The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.
    Type: Grant
    Filed: July 14, 2017
    Date of Patent: March 24, 2020
    Assignee: CRISTALIA PRODUTOS QUIMICOS FARMACEUTICOS LTDA.
    Inventors: Marcos Castanheira Alegria, Lucidio Cristovao Fardelone, Marina Baiochi Riboldi Delalana, Josef Ernst Thiemann, Spartaco Astolfi Filho, Roberto Carlos Debom Moreira, Ogari de Castro Pacheco
  • ANIMAL PRODUCT-FREE CULTURE MEDIUM FOR BACTERIA OF THE GENUS CLOSTRIDIUM AND A PROCESS FOR PRODUCING SUPERNATANT COMPRISING ONE OR MORE COLLAGENOLYTIC AND GELATINOLYTIC PROTEASES
    Publication number: 20180187144
    Abstract: The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.
    Type: Application
    Filed: July 14, 2017
    Publication date: July 5, 2018
    Inventors: Marcos Castanheira ALEGRIA, Lucidio Cristovao FARDELONE, Marina Baiochi Riboldi DELALANA, Josef Ernst THIEMANN, Spartaco ASTOLFI FILHO, Roberto Carlos Debom MOREIRA, Ogari de Castro PACHECO
  • Animal product-free culture medium and a process for producing a supernatant of clostridium comprising one or more collagenolytic and gelatinolytic proteases
    Patent number: 9725692
    Abstract: The present invention refers to a culture medium and a process for producing Clostridium collagenolytic and gelatinolytic proteases. The present invention includes an animal product-free culture medium for C. histolyticum comprising non-animal derived peptones, preferably vegetable derived peptones, yeast extract and the amino acids cysteine and arginine. The present invention also includes a process for producing a supernatant of Clostridium histolyticum liquid culture comprising one or more collagenolytic and gelatinolytic proteases, and pharmaceutical composition comprising as active ingredient a supernatant of the Clostridium cultures described herein.
    Type: Grant
    Filed: May 29, 2013
    Date of Patent: August 8, 2017
    Assignee: CRISTALIA PRODUTOS QUIMICOS FARMACEUTICOS LTDA.
    Inventors: Marcos Castanheira Alegria, Lucidio Cristovao Fardelone, Marina Baiochi Riboldi Delalana, Josef Ernst Thiemann, Spartaco Astolfi Filho, Roberto Carlos Debom Moreira, Ogari de Castro Pacheco
  • ANIMAL PRODUCT-FREE CULTURE MEDIUM FOR BACTERIA OF THE GENUS CLOSTRIDIUM AND A PROCESS FOR PRODUCING SUPERNATANT COMPRISING ONE OR MORE COLLAGENOLYTIC AND GELATINOLYTIC PROTEASES
    Publication number: 20150147313
    Abstract: The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.
    Type: Application
    Filed: May 29, 2013
    Publication date: May 28, 2015
    Inventors: Marcos Castanheira Alegria, Lucidio Cristoväo Fardelone, Marina Baiochi Riboldi Delalana, Josef Ernst Thiemann, Spartaco Astolfi Filho, Roberto Carlos Debom Moreira, Ogari de Castro Pacheco