Abstract: Methods and devices for generating ionized molecules for analysis in a mass spectrometer are provided. A device comprises a solid substrate having one or more edges for spray ionization, the substrate adapted for receiving extraction phase comprising molecules of interest. The solid substrate may comprise one or more indentations for receiving the extraction phase and desorption solvent. The indentation may extend to one of the edges of the substrate to channel the desorption solution to the edge for spray ionization. The solid substrate may comprise a magnetic portion for retaining magnetic extraction phase deposited thereon. The solid substrate itself may be free of any extraction phase prior to an extraction phase containing the molecules of interest being deposited thereon.

Abstract: Disclosed herein is a system for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device. This SPME device The system includes a desorption chamber containing solvent required for desorption of analytes from SPME device; a flow injector in fluid connection with the desorption chamber, the desorption chamber and the flow injector being fluidly connected by at least a flow-insulating fluid connector; a solvent source in fluid connection with the flow injector; and a fluid switch that: in a desorption position, allows the solvent to be sprayed from the flow injector while flow-insulating any desorption solution in the desorption chamber, and in an detecting position, turns off the solvent source while maintaining the fluid connection between the flow injector and the desorption chamber, transferring the desorption solution through the flow-insulating fluid connector to the flow injector as a substantially undiluted plug of liquid.

Abstract: Disclosed herein is a system for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device. This SPME device The system includes a desorption chamber containing solvent required for desorption of analytes from SPME device; a flow injector in fluid connection with the desorption chamber, the desorption chamber and the flow injector being fluidly connected by at least a flow-insulating fluid connector; a solvent source in fluid connection with the flow injector; and a fluid switch that: in a desorption position, allows the solvent to be sprayed from the flow injector while flow-insulating any desorption solution in the desorption chamber, and in an detecting position, turns off the solvent source while maintaining the fluid connection between the flow injector and the desorption chamber, transferring the desorption solution through the flow-insulating fluid connector to the flow injector as a substantially undiluted plug of liquid.