Patents by Inventor Marcus J. Horn

Marcus J. Horn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11174285
    Abstract: A method of collecting one or more target macromolecules in a capture membrane by gel electrophoresis is disclosed, as well as a kit for macromolecule isolation and recovery including: a preformed gel; a capture device; an insertion guide; and optionally, a migration gauge.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: November 16, 2021
    Assignee: Princeton Separations, Inc.
    Inventors: Paul T. Nix, Marcus J. Horn
  • Publication number: 20190389902
    Abstract: A method of collecting one or more target macromolecules in a capture membrane by gel electrophoresis is disclosed, as well as a kit for macromolecule isolation and recovery including: a preformed gel; a capture device; an insertion guide; and optionally, a migration gauge.
    Type: Application
    Filed: February 21, 2019
    Publication date: December 26, 2019
    Inventors: Paul T. Nix, Marcus J. Horn
  • Patent number: 8245740
    Abstract: A device for positioning a component thereof along x- and y-axes of the device is provided. The device includes first and second motors, a carriage containing the component to be positioned, a lead screw to position the carriage along a first axis of the device, and a keyed shaft to position component to be positioned along a second axis of the device. The first motor drives the lead screw and the second motor drives the flatted shaft. In some embodiments described herein, the flatted shaft turns a first pulley, which is connected to a second pulley by a belt. The component to be positioned is attached to the belt and moves along a second axis of the device as the belt moves.
    Type: Grant
    Filed: June 3, 2005
    Date of Patent: August 21, 2012
    Assignee: Alfa Wassermann, Inc.
    Inventors: Marcus J. Horn, Blaine Marsh
  • Publication number: 20080210646
    Abstract: The present invention is directed to a centrifuge rotor (10) having a rotor housing (14) with a substantially cylindrical in shape and having a substantially uniform opening extending through the center of the housing and along a length thereof, a rotor core (16) having a substantially cylindrical shape and sized and shaped to fit within the opening of the rotor housing. The rotor core further has at least two channels (30) in an outer surface thereof such that the channels, along with an interior wall of the rotor housing, define two sample spaces into which sample is delivered while the rotor is in operation.
    Type: Application
    Filed: June 3, 2005
    Publication date: September 4, 2008
    Inventor: Marcus J. Horn
  • Publication number: 20080190829
    Abstract: The present invention is directed to a device for positioning a component thereof along x- and y-axes of the device. The device includes first and second motors, a carriage containing the component to be positioned, a lead screw to position the carriage along a first axis of the device, and a keyed shaft to position component to be positioned along a second axis of the device. The first motor drives the lead screw and the second motor drives the flatted shaft. In some embodiments described herein, the flatted shaft turns a first pulley, which is connected to a second pulley by a belt. The component to be positioned is attached to the belt and moves along a second axis of the device as the belt moves.
    Type: Application
    Filed: June 3, 2005
    Publication date: August 14, 2008
    Inventors: Marcus J. Horn, Blaine Marsh
  • Patent number: 6427731
    Abstract: This invention is directed to a cassette chemical immobilization and treatment system and method for enabling the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns (“mini-columns”), reagent wells containeing pre-packaged reagents including powdered, micoencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment means for the analyte sample columns, and, a machine readable instruction code Bet for identifying a chemical treatment protocol. The mini-columns are improved columns having high pressure interface capability to permit direct insertion of the mini-column into high-pressure solvent line for use as a support column for HPLC analysis.
    Type: Grant
    Filed: December 13, 1999
    Date of Patent: August 6, 2002
    Assignee: Amersham Pharmacia Biotech AB
    Inventor: Marcus J. Horn
  • Patent number: 6173603
    Abstract: A cassette is used with a chemical immobilization and treatment system. The system with the cassette performs various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns (“mini-columns”), having a high-pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis. A sample loader loads analyte samples either singly or simultaneously into the multiple, addressable mini-columns without the need to load the sample funnel/column assembly into a separate reaction chamber.
    Type: Grant
    Filed: September 3, 1998
    Date of Patent: January 16, 2001
    Assignee: Amersham Pharmacia Biotech AB
    Inventor: Marcus J. Horn
  • Patent number: 6168760
    Abstract: This invention is directed to a cassette chemical chemical treatment station. The system with the cassette performs various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly having a high pressure interface capability to permit direct insertion of the mini-column into a high-pressre solvent line for use as a support column for HPLC analysis. A sample loader loads analyte samples either singly or simultaneously into the multiple, addressable mini-columns without the need to load the sample funnel/column assembly into a separate reaction chamber.
    Type: Grant
    Filed: February 25, 1999
    Date of Patent: January 2, 2001
    Assignee: Amersham Pharmacia Biotech AB
    Inventor: Marcus J. Horn
  • Patent number: 6153437
    Abstract: A cassette chemical immobilization and treatment method enables the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns ("mini-columns"), having a high-pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis. A sample loader loads analyte samples either singly or simultaneously into the multiple, addressable mini-columns without the need to load the sample funnel/column assembly into a separate reaction chamber.
    Type: Grant
    Filed: February 25, 1999
    Date of Patent: November 28, 2000
    Assignee: Amersham Pharmacia Biotech AB
    Inventor: Marcus J. Horn
  • Patent number: 6082417
    Abstract: This invention is directed to a cassette chemical immobilization and treatment system and method for enabling the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns ("mini-columns"), reagent wells containeing pre-packaged reagents including powdered, micoencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment means for the analyte sample columns, and a machine readable instruction code set for identifying a chemical treatment protocol. The mini-columns are improved columns having high pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis.
    Type: Grant
    Filed: September 3, 1998
    Date of Patent: July 4, 2000
    Inventor: Marcus J. Horn
  • Patent number: 6039924
    Abstract: This invention is directed to a cassette chemical immobilization and treatment system and method for enabling the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly having a high-pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis. A sample loader loads analyte samples either singly or simultaneously into the multiple, addressable mini-columns without the need to load the sample funnel/column assembly into a separate reaction chamber.
    Type: Grant
    Filed: September 3, 1998
    Date of Patent: March 21, 2000
    Assignee: Amersham Pharmacia Biotech AB
    Inventor: Marcus J. Horn
  • Patent number: 5918273
    Abstract: This invention is directed to a unitary one-piece sample mini-column for a cassette chemical immobilization and treatment system for enabling the performance of various complex chemistries. The cassette assembly comprises a plurality of analyte sample columns ("mini-columns"), reagent wells containing pre-packaged reagents including powdered, microencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment mechanisms for the analyte sample columns, and a machine readable instruction code set for identfying a chemical treatment protocol. The mini-columns are improved columns having a high pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis.
    Type: Grant
    Filed: February 20, 1997
    Date of Patent: June 29, 1999
    Inventor: Marcus J. Horn
  • Patent number: 5800784
    Abstract: This invention is directed to a cassette chemical immobilization and treatment system and method for enabling the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns ("mini-columns"), reagent wells containing pre-packaged reagents including powdered, micoencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment means for the analyte sample columns, and a machine readable instruction code set for identifying a chemical treatment protocol. The mini-columns are improved columns having high pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis.
    Type: Grant
    Filed: July 9, 1996
    Date of Patent: September 1, 1998
    Inventor: Marcus J. Horn
  • Patent number: 5077014
    Abstract: Apparatus for immobilizing proteins and large polypeptides on a carrier support for solid-phase sequence. Upon irradiation, a functional group bound to the carrier is excited to a triplet biradical state and thereby rendered capable of bonding to amino acid units; unbound functional groups return to their starting state upon removal of the radiation.
    Type: Grant
    Filed: June 7, 1990
    Date of Patent: December 31, 1991
    Assignee: Hewlett-Packard Company
    Inventor: Marcus J. Horn
  • Patent number: 5071529
    Abstract: A method for immobilizing proteins and large polypeptides on a carrier support for solid-phase sequence. Upon irradiation, a functional group bound to the carrier is excited to a triplet biradical state and thereby rendered capable of bonding to amino acid units; unbound functional groups return to their starting state upon removal of the radiation.
    Type: Grant
    Filed: August 10, 1988
    Date of Patent: December 10, 1991
    Assignee: Hewlett-Packard Company
    Inventor: Marcus J. Horn
  • Patent number: 5008205
    Abstract: A method of detecting and identifying amino acid derivatives produced by sequential cleavage of N-terminal residues from a polypeptide chain. The polypeptide is first reacted with an isothiocyanate to produce an N-terminal-thiocarbamyl polypeptide. This derivative is then cleaved to form a cyclic ATZ derivative, which is subsequently combined with a detectable alcohol in anhydrous acid to primarily produce a stable thiocarbamyl amino acid ester. This compound may be readily detected and identified.
    Type: Grant
    Filed: August 10, 1988
    Date of Patent: April 16, 1991
    Assignee: Hewlett-Packard Company
    Inventor: Marcus J. Horn
  • Patent number: 4800166
    Abstract: A method for monitoring a solid-phase peptide synthesis for free amino ends of unreacted peptide chains, the monitoring occurring at the end of each addition of a blocked amino acid to the peptide chains which are anchored to the solid phase. The method includes reacting the solid phase with a monitoring agent that forms a covalent bond with unblocked amino groups. The solid phase is then washed to remove the unreacted monitoring agent. A cleaving reagent is then used to selectively remove the covalently bound monitoring reagent from the ends of the unblocked peptide chains, while leaving the blocked chains intact. The amount of monitoring agent thus removed is then quantitatively measured to determine what proportion of the initial peptide chains failed to react with the blocked amino acid.Preferred monitoring agents for use in this process include trityl (triphenylmethyl) -based groups, and particularly preferred agents are trityl, monomethoxytrityl, dimethoxytrityl, and trimethoxytrityl chlorides.
    Type: Grant
    Filed: June 6, 1986
    Date of Patent: January 24, 1989
    Assignee: Applied Protein Technologies, Inc.
    Inventors: Marcus J. Horn, Joanne Recchia
  • Patent number: 4701304
    Abstract: A system maintained under a constant reference pressure for the automated synthesis of peptides includes a reaction vessel that has a single port for both injection and withdrawal of the various fluids used in the peptide synthesis sequence, a plurality of reservoirs for holding the amino acids used in the synthesis of the peptide chains and a plurality of reservoirs for holding the solvents and reagents used to promote the synthesis of the peptide chains. The system also includes a volume displacement pump for removing a controlled volume of gas from the reaction vessel as the first part of each injection step to reduce the pressure within the vessel. This is followed by connection of a selected reservoir to the reaction vessel and flow from the reservoir to the vessel resulting from the pressure differential between the vessel and the reservoir. As soon as the pressure in the reaction vessel has returned to reference level, the transfer is complete.
    Type: Grant
    Filed: April 19, 1985
    Date of Patent: October 20, 1987
    Assignee: Applied Protein Technologies, Inc.
    Inventors: Marcus J. Horn, William K. Miller
  • Patent number: 4155714
    Abstract: A process and apparatus for the conversion of anilinothiazolino amino acid derivatives of peptides and proteins for use with conventional sequencers in the overall Edman degradation process. The apparatus comprises a modular unit separate from the sequencer having an independently programmable control and flow actuating and directing means including a source of pressurized nitrogen, a conversion reagent container, a wash solvent container, a reaction vessel, and a plurality of interconnecting tubes and valves controlled so as to regulate the amounts and durations of all fluids entering and leaving said reaction vessel under gas pressure.The process comprises performing the steps of conversion, sample recovery, drying and washing at temperatures below but near to the respective boiling points of both the reagent and the solvent, and under reflux tower conditions.
    Type: Grant
    Filed: March 31, 1978
    Date of Patent: May 22, 1979
    Inventors: Alex G. Bonner, Marcus J. Horn
  • Patent number: 4153416
    Abstract: A process and apparatus for pulse labelling protein material for high sensitivitiy sequence analysis of protein material by Edman degradation technique comprising an isolated, auxiliary unit for storing a radioactive coupling reagent together with tubes, valves and controls for interrupting the Edman process, for pumping a measure of radioactive coupling reagent into the reaction vessel, a timer for controlling duration of contact between the radioactive reagent, and the protein material to maximize the coupling reaction therebetween, and thereafter reactivating the Edman process to admit nonradioactive coupling reagent to the vessel and to drive the coupling reaction to completion.
    Type: Grant
    Filed: June 6, 1977
    Date of Patent: May 8, 1979
    Inventors: Alex G. Bonner, Marcus J. Horn