Abstract: A method of performing acoustically enhanced sedimentation in a sample of a suspension comprising particles and a fluid is provided. The method comprises the steps of: i. introducing a volume of the sample into an accumulation zone of a microfluidic cavity; ii performing the substeps of: a. during a first time period: subjecting the volume to an acoustic standing wave configured to cause the particles in the volume to accumulate in at least a first region of the volume; b. during a second time period: subjecting the volume to a gravitational field affecting the particles and the fluid so that the particles accumulated in the at least one first region sediment in a first direction in relation to the direction of the gravitational field, thereby removing particles from the volume; and iii. moving the volume in a second direction opposite the first direction by introducing a subsequent volume of the sample into the accumulation zone of the microfluidic cavity. An acoustofluidic system is also provided.