Abstract: Provided are modified proteins comprising an introduced zinc finger DNA binding domain, and polynucleotides encoding the modified proteins. The modified proteins have a changed DNA binding location relative a DNA binding location of the protein in its unmodified form. The modified proteins include, in addition to the introduced zinc finger DNA binding domain, a gene expression activator domain or a gene expression repressor domain. Methods of using the modified proteins to activate or repress gene expression are also provided. The methods include prophylactic and therapeutic approaches.

Abstract: Provided are improved compositions and methods that are used for identifying interacting zinc fingers in a zinc finger and DNA sequence context. The compositions and methods provide a comprehensive approach that takes into account the effect of adjacent zinc fingers, in part by expanding the repertoire of F2 fingers that are varied at amino acid position 6.

Abstract: A system allows for rapid and specific induction of individual genes in eukaryotic cells using a chimeric transcriptional activator that is responsive to hormone inducer. Upon addition of the hormone, cytoplasmic transcriptional activator localizes to the nucleus and subsequently binds to promoters containing sequences that bind to its DNA-binding domain. Genetic modifications allow for rapid and specific degradation of a targeted protein upon addition of hormone by means of a regulated degron method that utilizes a protease variant. This system is useful for discovering new compounds by high throughput screening when introducing compound libraries to these protein-depleted cells.

Abstract: A system allows for rapid and specific induction of individual genes in eukaryotic cells using a chimeric transcriptional activator that is responsive to hormone inducer. Upon addition of the hormone, cytoplasmic transcriptional activator localizes to the nucleus and subsequently binds to promoters containing sequences that bind to its DNA-binding domain. Genetic modifications allow for rapid and specific degradation of a targeted protein upon addition of hormone by means of a regulated degron method that utilizes a protease variant. This system is useful for discovering new compounds by high throughput screening when introducing compound libraries to these protein-depleted cells.