Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

Abstract: The present invention relates to a method for producing a protein, novel nucleic acid fragment, an expression vector, and a method for enhancing the protein production in Pichia. The present invention provides that the combination of the modified signal peptide, increased copy number of the gene, and/or overexpressed Sec4p resulted in high level secretion of proteins.

Abstract: A recombinant protein is prepared comprising a polypeptide of interest and a starch binding domain (SBD). The said SBD is obtainable from glucoamylase of fungi genus Rhizopus. The said recombinant protein comprising the said SBD can be purified by contacting with an affinity matrix such as starch, the SBD binds to the affinity matrix to isolate the recombinant protein. The recombinant protein can be purified by separating the association between the SBD and the affinity matrix by acid, alkaline, salt, or sugar. The polypeptide of interest may be an antibody, an antigen, a therapeutic compound, an enzyme, or a protein and may apply in pathogen destruction, vaccine producing, and oral care product manufacturing. The SBD further provides as a tool to screen or identify polysacchrides.

Abstract: A recombinant protein is prepared comprising a polypeptide of interest and a starch binding domain (SBD). The said SBD is obtainable from glucoamylase of fungi genus Rhizopus. The said recombinant protein comprising the said SBD can be purified by contacting with an affinity matrix such as starch, the SBD binds to the affinity matrix to isolate the recombinant protein. The recombinant protein can be purified by separating the association between the SBD and the affinity matrix by acid, alkaline, salt, or sugar. The polypeptide of interest may be an antibody, an antigen, a therapeutic compound, an enzyme, or a protein and may apply in pathogen destruction, vaccine producing, and oral care product manufacturing. The SBD further provides as a tool to screen or identify polysacchrides.

Abstract: A carcass of a soccer ball or other inflatable game ball has a bladder with a valve for introducing air into said bladder; a fabric pocket layer located outside said bladder composed of a plurality of fabric pieces sewn to form a spherical shape, with a hole for the valve, the sewing thread for sewing said fabric pieces and the sewn margin being located in the inner side of said fabric pocket, and an opening being cut for reversing and placing the bladder being located on one of the fabric pieces; and an opening-cover adhered solidly on said opening for enclosing said opening. The surface of the manufactured ball is soft, with good tactility and the performance of ball-controlling is good. At the same time because there is no sewn margin left on the out side of the fabric pocket layer, no slight unevenness will exist on the surface of the fabric pocket layer, the ball will have a substantially perfect spherical shape, and can be easily controlled by athletes during a game.