Patents by Inventor Margaret Y. Insley

Margaret Y. Insley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Expression of factor VII and IX activities in mammalian cells
    Patent number: 8603777
    Abstract: Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIa or Factor IX. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII or Factor IX. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain, joined to a second nucleotide sequence positioned downstream of the first sequence. The second sequence encodes a catalytic domain for the serine protease activity of either Factor VIIa or Factor IX. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as either Factor VIIa or Factor IX.
    Type: Grant
    Filed: September 25, 1991
    Date of Patent: December 10, 2013
    Assignee: ZymoGenetrics, Inc.
    Inventors: Frederick S. Hagen, Mark J. Murray, Sharon J. Busby, Kathleen L. Berkner, Margaret Y. Insley, Richard G. Woodbury, Charles L. Gray
  • Expression of factor VII activity in mammalian cells
    Patent number: 4784950
    Abstract: Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIa. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain, joined to a second nucleotide sequence positioned downstream of the first sequence. In particular, the first nucleotide sequence may be derived from a genomic clone or cDNA clone of Factor VII. The second sequence encodes a catalytic domain for the serine protease activity of Factor VIIA. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as Factor VIIa.
    Type: Grant
    Filed: December 16, 1986
    Date of Patent: November 15, 1988
    Assignee: ZymoGenetics, Inc.
    Inventors: Frederick S. Hagen, Mark J. Murray, Sharon J. Busby, Kathleen L. Berkner, Margaret Y. Insley, Richard G. Woodbury, Charles L. Gray
  • Site specific mutagenesis in alpha-1-antitrypsin
    Patent number: 4711848
    Abstract: Methods are provided for producing sites specific mutagenized alpha-1-antitrypsin. Particular mutants are formed having a mutation in the active site of alpha-1-antitrypsin at amino acid position 358 and at amino acid position 342.
    Type: Grant
    Filed: March 7, 1985
    Date of Patent: December 8, 1987
    Assignee: ZymoGenetics, Inc.
    Inventors: Margaret Y. Insley, Glenn Kawasaki
  • Expression of tissue-type plasminogen activator in bacteria
    Patent number: H2055
    Abstract: A method, and DNA constructs useful therein, is provided for producing plasminogen activator in bacteria. Particular constructs are provided comprising a coding sequence for tissue plasminogen activator and the leader peptide thereof, which is used to transform E. coli to produce protein having activity of tissue plasminogen activator.
    Type: Grant
    Filed: September 20, 1985
    Date of Patent: December 3, 2002
    Assignee: ZymoGenetics, Inc.
    Inventors: Donald D. Rao, Margaret Y. Insley