Abstract: A method for the production of a functional prokaryotic membrane transporter protein in a eukaryotic host organism comprising the following steps: obtaining a DNA construct by ligating a DNA coding sequence of a prokaryotic membrane transporter protein to the N-terminal and/or C-terminal DNA coding sequences of a eukaryotic membrane protein; introducing the obtained DNA construct in the eukaryotic host organism for the production of the functional prokaryotic membrane transporter protein.

Abstract: An application of monoolein-based cationic lipid systems can be used for the complexation, transport and transfection of genetic material into target cells. The lipid systems can be prepared through the conjugation of the neutral lipid monooleoyl-rac glycerol (Monoolein) with the cationic tensioactives derived from Dioctadecyldimethylammonium (DODAX) as, for example, Dioctadecyldimethylammonium Bromide (DODAB), Dioctadecyldimethylammonium Chloride (DODAC) and Dioctadecyldimethylammonium Phosphate (DODAP). The lipofection method can present high levels of biocompatibility and reduced acquisition costs, and is applicable for molecular biology purposes (in vitro application), as well as in the health area in gene therapy (in vivo application).