Patents by Inventor Margo R. MONROE

Margo R. MONROE has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNA-guided gene editing and regulation
    Patent number: 11746349
    Abstract: Methods and compositions of altering a eukaryotic cell are described including providing to the eukaryotic cell a guide DNA sequence complementary to a target nucleic acid sequence, providing to the eukaryotic cell an Ago enzyme or a nuclease null Ago protein that interacts with the guide DNA sequence for DNA-guided gene editing and regulation of the target nucleic acid sequence in a site specific manner.
    Type: Grant
    Filed: February 7, 2017
    Date of Patent: September 5, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Luhan Yang, Margo R. Monroe, Po-Yi Huang
  • Mitochondrial genome editing and regulation
    Patent number: 11208652
    Abstract: Methods and compositions of altering mitochondrial DNA of a eukaryotic cell are provided using one or more of a mitochondrial specific adeno-associated virus to deliver one or more nucleic acids encoding CRISPR system including a Cas9 protein or its nuclease inactive variant and a guide RNA into a mitochondria for expression within the mitochondria. The Cas9 system can cut, nick or regulate a target mitochondrial nucleic acid.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: December 28, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Luhan Yang, Margo R. Monroe
  • DNA-Guided Gene Editing and Regulation
    Publication number: 20210189388
    Abstract: Methods and compositions of altering a eukaryotic cell are described including providing to the eukaryotic cell a guide DNA sequence complementary to a target nucleic acid sequence, providing to the eukaryotic cell an Ago enzyme or a nuclease null Ago protein that interacts with the guide DNA sequence for DNA-guided gene editing and regulation of the target nucleic acid sequence in a site specific manner.
    Type: Application
    Filed: February 7, 2017
    Publication date: June 24, 2021
    Applicants: President and Fellows of Harvard College, President and Fellows of Harvard College
    Inventors: George M. Church, Luhan Yang, Margo R. Monroe, Po-Yi Huang
  • Mitochondrial Genome Editing and Regulation
    Publication number: 20190062739
    Abstract: Methods and compositions of altering mitochondrial DNA of a eukaryotic cell are provided using one or more of a mitochondrial specific adeno-associated virus to deliver one or more nucleic acids encoding CRISPR system including a Cas9 protein or its nuclease inactive variant and a guide RNA into a mitochondria for expression within the mitochondria. The Cas9 system can cut, nick or regulate a target mitochondrial nucleic acid.
    Type: Application
    Filed: February 2, 2017
    Publication date: February 28, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: George M. Church, Luhan Yang, Margo R. Monroe
  • Nanoparticles for self referencing calibration
    Patent number: 10151680
    Abstract: Systems and methods for high-throughput processing of assay plates include a calibration nanoparticle to facilitate automated focusing of the imaging system. An assay plate includes a base layer, a transparent layer in contact with the base layer, and at least one calibration nanoparticle having a pre-defined size immobilized on the assay plate surface. The assay plate surface can be functionalized to selectively bind to biological targets. The assay plate can be used in an imaging system for high-throughput autofocus and biological target detection.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: December 11, 2018
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Selim M. Unlu, George Daaboul, Margo R. Monroe, Carlos Lopez, Ahmet Tuysuzoglu, Sunmin Ahn
  • NANOPARTICLES FOR SELF REFERENCING CALIBRATION
    Publication number: 20160282253
    Abstract: Systems and methods for high-throughput processing of assay plates include a calibration nanoparticle to facilitate automated focusing of the imaging system. An assay plate includes a base layer, a transparent layer in contact with the base layer, and at least one calibration nanoparticle having a pre-defined size immobilized on the assay plate surface. The assay plate surface can be functionalized to selectively bind to biological targets. The assay plate can be used in an imaging system for high-throughput autofocus and biological target detection.
    Type: Application
    Filed: October 28, 2014
    Publication date: September 29, 2016
    Inventors: Selim M. UNLU, George DAABOUL, Margo R. MONROE, Carlos LOPEZ, Ahmet TUYSUZOGLU