Abstract: The present invention relates to a vaccine for use in therapeutic vaccination of a human subject against a human papillomavirus 16 (HPV16)-related virus, wherein said vaccine provides at least five discrete immunization peptides consisting of amino acid sequences selected from at least five of the following groups: (i) SEQ ID NOs: 1 to 10; (ii) SEQ ID NOs: 11 to 52; (iii) SEQ ID NOs:53 to 79; (iv) SEQ ID NOs: 71 and 80 to 96; (v) SEQ ID NOs:25, 81, and 97-106; and (vi) SEQ ID NOs: 9, 12, 23, 63, 81, 85, 101, and 107 to 131; wherein said vaccine is a human leukocyte antigen (HLA) universal vaccine for use in any human subject expressing at least one HLA of a HLA supertype selected from the list consisting of HLA-A01, HLA-A02, HLA-A03/A11, HLA-A24, HLA-B07, and HLA-B15, and wherein each of said at least five immunization peptides, when presented as an H LA-complex, activates human anti-immunization peptide T cells. The present invention also relates to kits, devices, and methods related thereto.

Abstract: The present invention relates to an in vitro method for producing an immunoreactive agent against human cancer cells infected with HPV16-related virus, said method comprising expressing an at least partial nucleic acid sequence encoding an immunoreactive agent obtained from an immune cell stimulated by a complex comprising a human leukocyte antigen (HLA) and a stimulation peptide (stimulation complex), wherein said stimulation peptide (i) consists of an amino acid sequence selected from SEQ ID NOs: 1 and 2 and said HLA is from HLA supertype HLA-A01; (ii) consists of an amino acid sequence of SEQ ID NO: 11 and said HLA is from HLA supertype HLA-A02, (iii) consists of an amino acid sequence selected from SEQ ID NOs:34 to 37 and said HLA is from HLA supertype HLA-A03/A11; (iv) consists of an amino acid sequence selected from SEQ ID NOs:60 and bland said HLA is from HLA supertype HLA-A24; (v) consists of an amino acid sequence selected from SEQ ID NOs:61, 76, and 77 and said HLA is from HLA supertype HLA-B07; or