Patents by Inventor Maria D. Cebezas

Maria D. Cebezas has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cellular assays with a molecular endpoint measured by SAMDI mass spectrometry
    Patent number: 11047861
    Abstract: The disclosure provides a cell-based, label-free assay compatible with high-throughput screening (HTS) that can report quantitatively on enzyme activities by measuring mass changes of substrates with MALDI-mass spectrometry.
    Type: Grant
    Filed: August 8, 2016
    Date of Patent: June 29, 2021
    Assignee: NORTHWESTERN UNIVERSITY
    Inventors: Milan Mrksich, Eric J. Berns, Maria D. Cebezas
  • Nanopatterned extracellular matrices enable cell-based assays with a mass spectrometric readout
    Patent number: 10563244
    Abstract: The present disclosure provides methods in which adherent cells are treated with small molecules, cultured, lysed, and then analyzed by mass spectrometry to measure the activities of endogenous enzymes. The implementation of this method relies on the use of surfaces that are nanopatterned with cell adhesion ligands to mediate cell attachment and a peptide that is a substrate for the desired enzyme activity in the lysate.
    Type: Grant
    Filed: September 20, 2017
    Date of Patent: February 18, 2020
    Assignee: NORTHWESTERN UNIVERSITY
    Inventors: Milan Mrksich, Maria D. Cebezas, Chad A. Mirkin
  • CELLULAR ASSAYS WITH A MOLECULAR ENDPOINT MEASURED BY SAMDI MASS SPECTROMETRY
    Publication number: 20180231564
    Abstract: The disclosure provides a cell-based, label-free assay compatible with high-throughput screening (HTS) that can report quantitatively on enzyme activities by measuring mass changes of substrates with MALDI-mass spectrometry.
    Type: Application
    Filed: August 8, 2016
    Publication date: August 16, 2018
    Inventors: Milan Mrksich, Eric J. Berns, Maria D. Cebezas
  • NANOPATTERNED EXTRACELLULAR MATRICES ENABLE CELL-BASED ASSAYS WITH A MASS SPECTROMETRIC READOUT
    Publication number: 20180080058
    Abstract: The present disclosure provides methods in which adherent cells are treated with small molecules, cultured, lysed, and then analyzed by mass spectrometry to measure the activities of endogenous enzymes. The implementation of this method relies on the use of surfaces that are nanopatterned with cell adhesion ligands to mediate cell attachment and a peptide that is a substrate for the desired enzyme activity in the lysate.
    Type: Application
    Filed: September 20, 2017
    Publication date: March 22, 2018
    Inventors: Milan Mrksich, Maria D. Cebezas, Chad A. Mirkin