Abstract: A kit and method to perform a biological assay for evaluating the bioleaching potential of minerals, which allows to know the metallurgic response of minerals for geo-mining-metallurgic short and mid term planning, wherein the kit comprises a propylene tube-type container which has a line that specifies the liquid filling level, where is it possible to place a propylene sphere that floats at the liquid level and it has a plastic twist off cup on the upper extreme, while in the lower extreme or base there is a lyophilized biomass (powder) in charge of the mineral leaching.